A NMR-based, non-targeted multistep metabolic profiling revealed L-rhamnitol as a metabolite that characterised apples from different geographic origins
A NMR-based, non-targeted multistep metabolic profiling revealed L-rhamnitol as a metabolite that characterised apples from different geographic origins
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DOI：
10.1016/j.foodchem.2014.11.028
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发表时间：
2015-05-01
期刊：
影响因子：
8.8
通讯作者：
Sekiyama, Yasuyo
中科院分区：
文献类型：
--
作者：
Tomita, Satoru;Nemoto, Tadashi;Sekiyama, Yasuyo
这项研究利用基于H-1核磁共振的代谢图谱来描述生长在日本(富士、奥林和乔纳戈德)或新西兰(富士、爵士和嫉妒)的五个品种的苹果。主成分分析(PCA)显示富士-Orin-Jonagold类和Jazz-Envy类之间存在明显的分离，主要对应于蔗糖、葡萄糖和果糖等糖信号的差异。多步主成分分析去除了主要糖类的影响，突出了次要代谢物，如天冬氨酸、2-甲基苹果酸和一种未知化合物。这些次要代谢产物根据不同的地理区域将苹果分为两类。随后的偏最小二乘判别分析(偏最小二乘判别分析)表明了未知代谢物的重要性。该代谢物经木炭层析分离，经二维核磁共振和LC/MS鉴定为L鼠李糖醇。L鼠李醇对地理区分的显著贡献表明，苹果可以根据不同的因素进行区分，包括储藏期、栽培方法和气候。(C)2014爱思唯尔有限公司。保留所有权利。
This study utilises H-1 NMR-based metabolic profiling to characterise apples of five cultivars grown either in Japan (Fuji, Orin, and Jonagold) or New Zealand (Fuji, Jazz, and Envy). Principal component analysis (PCA) showed a clear separation between the Fuji-Orin-Jonagold class and the Jazz-Envy class, primarily corresponding to the differences in sugar signals, such as sucrose, glucose, and fructose. Multistep PCA removed the influence of dominant sugars and highlighted minor metabolites such as aspartic acid, 2-methylmalate, and an unidentified compound. These minor metabolites separated the apples into two classes according to different geographical areas. Subsequent partial least squares discriminant analysis (PLS-DA) indicated the importance of the unidentified metabolite. This metabolite was isolated using charcoal chromatography, and was identified as L-rhamnitol by 2D NMR and LC/MS analyses. The remarkable contribution of L-rhamnitol to geographic discrimination suggests that apples may be characterised according to various factors, including storage duration, cultivation method, and climate. (C) 2014 Elsevier Ltd. All rights reserved.