AtINO80 and AtARP5 physically interact and play common as well as distinct roles in regulating plant growth and development
AtINO80 and AtARP5 physically interact and play common as well as distinct roles in regulating plant growth and development
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AtINO80 和 AtARP5 发生物理相互作用，在调节植物生长和发育中发挥共同和独特的作用
DOI：
10.1111/nph.15780
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发表时间：
2019-07-01
期刊：
影响因子：
9.4
通讯作者：
Zhu, Yan
中科院分区：
文献类型：
--
作者：
Kang, Huijia;Zhang, Chi;Zhu, Yan
染色质结构的适当调节依赖于染色质重塑因子的活性及其相互作用。在这里，我们表明，拟南芥染色质重塑AtINO 80与肌动蛋白相关蛋白AtARP 5相互作用，可以形成一个更大的蛋白质复合物。遗传分析表明，AtARP 5与AtINO 80在植物细胞增殖和复制应激反应中协同作用。同时，AtARP 5在AtINO 80介导的开花时间调控及相关转录调控中并不必需，它们在开花抑制基因FLC/MAF 4/MAF 5区域的染色质分布模式也不同。体外DNase I消化试验显示AtINO 80 N-末端可以弱结合DNA，这种相互作用被H2A.Z/H2 B添加显著抑制。AtARP 6是SWR 1-C的一个特异性亚基，介导H2A.Z交换，被发现在AtINO 80的局部染色质富集中具有先前意想不到的抑制作用。进一步的遗传分析揭示了AtINO 80和AtARP 6之间的功能相互作用及其在胚胎发生和胚后器官发育中的关键作用，以及AtARP 5和AtARP 6在维持基因组稳定性方面的协同作用。我们的研究结果为AtINO 80和AtARP 5在植物发育的各个方面的共同和独特作用提供了见解。
The proper modulation of chromatin structure is dependent on the activities of chromatin-remodeling factors and their interplays. Here, we show that the Arabidopsis chromatin-remodeler AtINO80 interacts with the actin-related protein AtARP5 and can form a larger protein complex. Genetic analysis demonstrated that AtARP5 acts in concert with AtINO80 during plant cellular proliferation and replication stress response. At the same time, AtARP5 is not required for AtINO80-mediated control of flowering time and related transcriptional regulation, and their chromatin distribution patterns on regions of flowering-repressor genes FLC/MAF4/MAF5 are also different. An in vitro DNase I digestion assay revealed that the AtINO80N-terminus can weakly bind DNA, an interaction that is significantly inhibited by H2A.Z/H2B addition. AtARP6, a specific subunit of SWR1-C that mediates the H2A.Z exchange, was found to have a previously unexpected inhibitory role in the local chromatin enrichment of AtINO80. Further genetic analyses revealed the functional interplay between AtINO80 and AtARP6 and their critical roles in embryogenesis and post-embryonic organ development, as well as the synergy of AtARP5 and AtARP6 in maintaining genomic stability. Our findings provide insights into the common and distinct roles of AtINO80 and AtARP5 in diverse aspects of plant development.