In vivo X-ray angiography in the mouse brain using synchrotron radiation
In vivo X-ray angiography in the mouse brain using synchrotron radiation
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DOI：
10.1161/01.str.0000226904.96059.a6
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发表时间：
2006-07-01
期刊：
影响因子：
8.3
通讯作者：
Umetani, Keiji
中科院分区：
文献类型：
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作者：
Kidoguchi, Keiji;Tamaki, Masahiro;Umetani, Keiji
背景和目的——我们首次使用第三代同步辐射设备SPring-8对小鼠大脑进行活体X射线血管造影。方法——将细PE-50管置于成年雄性C57BL/6J小鼠的单侧颈外动脉中。在维持颈内动脉血流的同时，注射33μL造影剂，然后进行半球选择性血管造影。 结果-大脑动脉平均直径如下：大脑中动脉142.5±7.90μm，大脑前动脉138.3±9.35μm，大脑后动脉120.5±5.53μm，颈内动脉 162.6 +/- 10.87 μm (n = 5)。为了证明直径的变化，我们诱导高碳酸血症并检测到血管扩张范围为原始直径的 121% 至 124% (n = 5)。我们还在颈动脉内注射血管舒张药物盐酸罂粟碱、ATP二钠和盐酸法舒地尔水合物前后对小鼠进行了重复血管造影，并显示了注射后1、5、15和30分钟每条动脉直径的时间变化（每种药物n = 1）。结论-仅使用最少量的造影剂，同步辐射使我们能够研究X射线小鼠大脑血管造影。在生理条件下可以清楚地观察血管的形态。直径及其变化也可以在体内成功研究。
Background and Purpose-We, for the first time, performed in vivo x-ray angiography in the mouse brain using SPring-8, a third-generation synchrotron radiation facility.Methods-A thin PE-50 tube was placed in the unilateral external carotid artery in adult male C57BL/6J mice. While maintaining the blood flow in the internal carotid artery, 33 mu L of contrast agent was injected and then selective angiography of the hemisphere was performed.Results-The average diameters of cerebral artery were as follows: 142.5 +/- 7.90 mu m in middle cerebral artery, 138.3 +/- 9.35 mu m in anterior cerebral artery, 120.5 +/- 5.53 mu m in posterior cerebral artery, and 162.6 +/- 10.87 mu m in internal carotid artery (n = 5). To demonstrate the changes in diameter, we induced hypercapnia and detected the dilatation of the vessels between 121% and 124% of the original diameters (n = 5). We also repeated angiography in the mice before and after intracarotid injection of vasodilatation drugs papaverine hydrochloride, ATP disodium, and fasudil hydrochloride hydrate and demonstrated the chronological changes in the diameters in each artery at 1, 5, 15, and 30 minutes after injection (n = 1 for each drug).Conclusions-Using only a minimum volume of the contrast agent, synchrotron radiation enables us to study x-ray angiography in the mouse brain. The morphology of the vessels can be clearly observed under physiological conditions. The diameters and their changes can also be successfully studied in vivo.