Biochemical characterization of pediatric brain tumors by using in vivo and ex vivo magnetic resonance spectroscopy
Biochemical characterization of pediatric brain tumors by using in vivo and ex vivo magnetic resonance spectroscopy
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DOI：
10.3171/jns.2002.96.6.1023
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发表时间：
2002-06-01
影响因子：
4.1
通讯作者：
Black, PM
中科院分区：
文献类型：
--
作者：
Tzika, AA;Cheng, LL;Black, PM
目的。磁共振 (MR) 波谱提供有关肿瘤的生化信息。作者试图通过使用磁共振波谱来确定儿科脑肿瘤的体内和离体生化特征之间的关系。他们的假设是离体 MR 光谱提供了体内 MR 光谱和神经病理学分析之间的联系。方法。在神经上皮肿瘤患者的手术前进行体内质子磁共振波谱分析。在切除过程中，从体内 MR 波谱识别的感兴趣体积内获得了总共 40 个肿瘤活检样本，并立即冷冻在液氮中。使用高分辨率魔角旋转 (HRMAS) 对这 40 个肿瘤活检样本进行离体 MR 波谱分析。使用相同的活检样本进行神经病理学分析，将肿瘤分类为室管膜瘤、脉络丛癌、松果体母细胞瘤（各一例）、毛细胞性星形细胞瘤、髓母细胞瘤、低级别胶质瘤和多形性胶质母细胞瘤（各两例）。 与体内 MR 光谱相比，离体 HRMAS MR 光谱改善了光谱中的线宽和线形。体内检测到的胆碱 (Cho) 对应于体外的三个不同峰（甘油磷酸胆碱、磷酸胆碱 [PCho] 和 Cho）。体内光谱的代谢物比率与离体光谱的比率相关（皮尔逊相关系数范围 r = 0.72-0.91；p 小于或等于 0.01）。离体光谱中的代谢物比率，例如 PCho/总肌酸 (tCr) 和脂质/tCr，分别与癌组织百分比和肿瘤坏死百分比相关（r = 0.84；p < 0.001）。结论。体内和离体 MR 光谱之间的一致性表明，离体 HRMAS MR 光谱可以提高这种模式的分辨率，并提供体内 MR 光谱和神经病理学分析之间的联系。
Object. Magnetic resonance (MR) spectroscopy provides biochemical information about tumors. The authors sought to determine the relationship between in vivo and ex vivo biochemical characterization of pediatric brain tumors by using MR spectroscopy. Their hypothesis was that ex vivo MR spectroscopy provides a link between in vivo MR spectroscopy and neuropathological analysis.Methods. In vivo proton MR spectroscopy was performed before surgery in I I patients with neuroepithelial tumors. During resection, a total of 40 tumor biopsy samples were obtained from within the volume of interest identified on in vivo MR spectroscopy and were frozen immediately in liquid nitrogen. High-Resolution Magic Angle Spinning (HRMAS) was used to perform ex vivo MR spectroscopy in these 40 tumor biopsy samples. Neuropathological analysis was performed using the same biopsy samples, and the tumors were classified as ependymoma, choroid plexus carcinoma, pineoblastoma (one each), and pilocytic astrocytoma, medullobastoma, low-grade glioma, and glioblastoma multiforme (two each).Ex vivo HRMAS MR spectroscopy improved line widths and line shapes in the spectra, compared with in vivo MR spectroscopy. Choline (Cho) detected in vivo corresponded to three different peaks ex vivo (glycerophosphocholine, phosphocholine [PCho], and Cho). Metabolite ratios from in vivo spectra correlated with ratios from ex vivo spectra (Pearson correlation coefficient range r = 0.72-0.91; p less than or equal to 0.01). Metabolite ratios from ex vivo spectra, such as PCho/total creatine (tCr) and lipid/tCr, correlated with the percentage of cancerous tissue and percentage of tumor necrosis, respectively (r = 0.84; p < 0.001).Conclusions. Agreement between in vivo and ex vivo MR spectroscopy indicates that ex vivo HRMAS MR spectroscopy can improve resolution of this modality and provide a link between in vivo MR spectroscopy and neuropathological analysis.