Altered long noncoding RNA expression profiles in the myocardium of rats with ischemic heart failure
Altered long noncoding RNA expression profiles in the myocardium of rats with ischemic heart failure
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DOI：
10.2459/jcm.0b013e32836499cd
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发表时间：
2015-07-01
影响因子：
3
通讯作者：
Wang, Lian-Sheng
中科院分区：
文献类型：
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作者：
Gao, Wei;Wang, Ze-Mu;Wang, Lian-Sheng
尽管冠心病的治疗取得了显著进展，但缺血性心力衰竭的患病率仍在迅速增加。长链非编码RNA是一类新的基因调控因子，可能与疾病的发生有关。本研究的目的是探讨长链非编码RNA的表达谱及其潜在的功能作用在缺血性heartfault.Methods我们应用了一个良好的缺血性心力衰竭大鼠模型，并进行长链非编码RNA芯片实验对缺血性心力衰竭大鼠和假手术对照组的左心室组织。通过倍数变化过滤鉴定差异表达的长非编码RNA和mRNA。结果在心力衰竭组中，有1197条长链非编码RNA和2066条mRNA表达上调，而1403条长链非编码RNA和2871条mRNA表达下调（变化倍数>2.0）。我们还鉴定了331对差异表达的长链非编码RNA和邻近的编码基因，其中包括291个长链非编码RNA和296个mRNA。结论本研究发现一组长链非编码RNA在缺血性心力衰竭大鼠中异常表达，可能参与缺血性心力衰竭的发病机制。我们的研究结果可能为更好地理解缺血性心力衰竭的分子基础提供一个新的视角。
Aims Despite significant advances in the treatment of coronary artery disease, the prevalence of ischemic heart failure is still increasing rapidly. Long noncoding RNAs are a novel class of gene regulators and may contribute to disease cause. The aim of the present study was to investigate the expression profiles of long noncoding RNAs and their potential functional roles in ischemic heart failure.Methods We applied a well-established ischemic heart failure rat model and performed long noncoding RNA microarray experiments on the left ventricular tissue of rats with ischemic heart failure and under sham control. Differentially expressed long noncoding RNAs and mRNAs were identified through fold-change filtering. Bioinformatic analyses were performed to predict the potential biological roles of key long noncoding RNAs.Results We found that 1197 long noncoding RNAs and 2066 mRNAs were upregulated, whereas 1403 long noncoding RNAs and 2871 mRNAs were downregulated in failing hearts (fold-change>2.0). We also identified 331 pairs of differentially expressed long noncoding RNAs and nearby coding genes, which contained 291 long noncoding RNAs and 296 mRNAs. Expression levels of four long noncoding RNA-mRNA pairs, which might be involved in the pathogenesis of ischemic heart failure were confirmed by quantitative real-time PCR.Conclusion Our study identified a set of long noncoding RNAs that were aberrantly expressed in rats with ischemic heart failure and might be involved in the pathogenesis of ischemic heart failure. The results of our study may provide a novel perspective for better understanding the molecular basis of ischemic heart failure.