The effect of cigarette smoking on the oral and nasal microbiota.
The effect of cigarette smoking on the oral and nasal microbiota.
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DOI：
10.1186/s40168-016-0226-6
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发表时间：
2017-01-17
期刊：
影响因子：
15.5
通讯作者：
Caporaso NE
中科院分区：
文献类型：
--
作者：
Yu G;Phillips S;Gail MH;Goedert JJ;Humphrys MS;Ravel J;Ren Y;Caporaso NE
这项研究的目的是调查吸烟是否会改变口腔和鼻部微生物的多样性、组成和结构。招募了23名吸烟者和20名从不吸烟者。从每个受试者身上采集9个样本，包括牙菌斑刮痕、唾液、口腔5个软组织部位的拭子和两个前鼻孔的1个鼻拭子。对16S rRNA V3-V4区进行了微生物图谱测序。我们发现，吸烟者口腔粘膜的α多样性低于不吸烟者，但在其他样本部位，吸烟状态对微生物多样性和组成没有显著影响。8个口腔部位的微生物谱有显著差异。这项研究调查了吸烟对口腔不同部位的影响，并显示了吸烟对口腔黏膜微生物区系的潜在影响。我们发现，当面临吸烟等环境扰动时，口腔微生物生态系统的显着异质性可能有助于大多数地点口腔微生物区系的稳定。本文的在线版本(doi：10.1186/s4016802266)包含补充材料，可供授权用户使用。
The goal of the study was to investigate whether cigarette smoking alters oral and nasal microbial diversity, composition, and structure. Twenty-three current smokers and 20 never smokers were recruited. From each subject, nine samples including supra and subgingiva plaque scrapes, saliva, swabs from five soft oral tissue sites, and one nasal swab from both the anterior nares were collected. 16S rRNA V3-V4 region was sequenced for microbial profiles. We found that alpha diversity was lower in smokers than in nonsmokers in the buccal mucosa, but in other sample sites, microbial diversity and composition were not significantly different by smoking status. Microbial profiles differed significantly among eight oral sites. This study investigates the effect of cigarette smoking on different sites of the oral cavity and shows a potential effect of cigarette smoking on the buccal mucosa microbiota. The marked heterogeneity of the oral microbial ecosystem that we found may contribute to the stability of the oral microbiota in most sites when facing environmental perturbations such as that caused by cigarette smoking. The online version of this article (doi:10.1186/s40168-016-0226-6) contains supplementary material, which is available to authorized users.