Molecular cloning, sequencing, and tissue and developmental expression of mouse cartilage oligomeric matrix protein (COMP)
Molecular cloning, sequencing, and tissue and developmental expression of mouse cartilage oligomeric matrix protein (COMP)
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DOI：
10.1002/jor.1100180412
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发表时间：
2000-07-01
影响因子：
2.8
通讯作者：
Di Cesare, PE
中科院分区：
文献类型：
--
作者：
Fang, C;Carlson, CS;Di Cesare, PE
采用逆转录-聚合酶链反应技术克隆了小鼠软骨寡聚基质蛋白cDNA并进行了序列测定。开放阅读框编码755个氨基酸的产物，该产物与大鼠和人软骨寡聚基质蛋白具有高度同一性并具有其所有特征性分子特征。这表明软骨寡聚基质蛋白在进化过程中是高度保守的。该克隆分别与人、牛和大鼠软骨寡聚基质蛋白cDNA具有83%、84%和95%的同一性。在成年小鼠的组织中，软骨寡聚基质蛋白不仅在软骨和肌腱中表达，而且在气管、骨、骨骼肌、眼、心脏和胎盘中也表达，在其他组织中未发现表达。免疫组化显示，软骨寡聚基质沉积早在10天后交配的预分化小鼠胚胎间充质。在第13天，在胚胎的所有软骨组织和骨骼肌中检测到。随着发育的进行，软骨寡聚基质蛋白的积累在生长板中是显著的。在性交后19天，它显著沉积在生长板、软骨膜和骨膜的肥大区以及关节软骨表面的浅层中，但在骺软骨的更中心区域中不存在。软骨寡聚基质蛋白在发育和成年小鼠组织中的有限组织分布和表达表明该蛋白在转录水平上的调节。本文报道的发现是第一个详细的表征软骨寡聚基质蛋白在小鼠早期骨骼发育过程中的分布。
Mouse cartilage oligomeric matrix protein cDNA was cloned and sequenced by a reverse transcription-polymerase chain reaction. The open reading frame encoded a product of 755 amino acids that shares a high degree of identity to and possesses all the characteristic molecular features of both rat and human cartilage oligomeric matrix protein. This suggests that cartilage oligomeric matrix protein is highly conserved during evolution. The clone was 83, 84, and 95% identical to human, bovine, and rat cartilage oligomeric matrix protein cDNA, respectively. In tissues from the adult mouse, cartilage oligomeric matrix protein was expressed not only in cartilage and tendon but in trachea, bone, skeletal muscle, eye, heart, and placenta as well, and no expression was found in other tissues. Immunohistology revealed that cartilage oligomeric matrix was deposited as early as 10 days post coitus in predifferentiated mouse embryo mesenchyme. It was detected in all cartilaginous tissues and in the skeletal muscles of the embryo at day 13. As development progressed, accumulation of cartilage oligomeric matrix protein was marked in the growth plate. At 19 days post coitus, it was prominently deposited in the hypertrophic zone of the growth plate, perichondrium, and periosteum and in the superficial layer of the articular cartilage surface but was absent in the more central areas of the epiphyseal cartilage. The restricted tissue distribution and expression of cartilage oligomeric matrix protein in developing as well as adult mouse tissues suggest the regulation of this protein at the transcriptional level. The findings reported herein are the first detailed characterization of the distribution of cartilage oligomeric matrix protein during early skeletal development of the mouse.