Production of latent collagenase by human umbilical vein endothelial cells in response to angiogenic preparations.
Production of latent collagenase by human umbilical vein endothelial cells in response to angiogenic preparations.
复制标题
人脐静脉内皮细胞响应血管生成制剂产生潜在胶原酶。
DOI：
10.1016/0014-4827(85)90545-2
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
Jaffe,EA
中科院分区：
文献类型：
--
作者：
Moscatelli,DA;Rifkin,DB;Jaffe,EA
测试了三种已知在体内是血管生成的并且刺激培养的牛毛细血管内皮（BCE）细胞产生潜伏胶原酶的制剂刺激培养的人脐静脉内皮（HUVE）细胞产生潜伏胶原酶的能力。牛视网膜提取物和鼠脂肪细胞条件培养基在有效刺激BCE细胞产生潜伏性胶原酶的浓度下对HUVE细胞产生潜伏性胶原酶没有影响。然而，用更高浓度的牛视网膜提取物，刺激HUVE细胞产生潜伏的胶原酶。人肝癌细胞超声处理物以剂量依赖性方式刺激HUVE细胞产生潜伏性胶原酶。刺激HUVE细胞产生潜伏胶原酶的人肝癌细胞超声处理物的浓度低于刺激BCE细胞产生潜伏胶原酶的有效浓度。HUVE细胞产生纤溶酶原激活物不受人肝癌细胞超声处理的影响。不同浓度的血清在HUVE文化并没有影响刺激潜伏的胶原酶生产的人肝癌细胞超声波处理，这表明血清成分既不阻止也不刺激胶原酶诱导因子的作用。虽然人肝癌细胞超声处理的报告，刺激内皮细胞增殖，纯化和部分纯化的内皮细胞有丝分裂原对潜在的胶原酶的生产没有影响。因此，至少两种含有血管生成活性的制剂将刺激HUVE细胞产生潜伏的胶原酶。
Three preparations known to be angiogenic in vivo and which stimulate production of latent collagenase by cultured bovine capillary endothelial (BCE) cells were tested for their ability to stimulate production of latent collagenase by cultured human umbilical vein endothelial (HUVE) cells. Bovine retinal extract and murine adipocyte-conditioned medium had no effect on production of latent collagenase by HUVE cells at concentrations that were effective in stimulating production of latent collagenase by BCE cells. However, with higher concentrations of bovine retinal extract, production of latent collagenase by HUVE cells was stimulated. Human hepatoma cell sonicate stimulated production of latent collagenase by HUVE cells in a dose-dependent manner. The concentration of human hepatoma cell sonicate which stimulated production of latent collagenase by HUVE cells was lower than the concentration that was effective for the stimulation of production of latent collagenase by BCE cells. Plasminogen activator production by HUVE cells was unaffected by human hepatoma cell sonicate. Varying the concentration of serum in HUVE cultures did not affect the stimulation of latent collagenase production by human hepatoma cell sonicate, suggesting that serum components neither block nor stimulate the action of the collagenase-inducing factor. Although human hepatoma cell sonicate is reported to stimulate endothelial cell multiplication, purified and partially purified endothelial cell mitogens had no effect on production of latent collagenase. Thus, at least two preparations which contain angiogenic activity will stimulate production of latent collagenase by HUVE cells.