Graphene oxide disrupts the protein-protein interaction between Neuroligin/NLG-1 and DLG-1 or MAGI-1 in nematode Caenorhabditis elegans
Graphene oxide disrupts the protein-protein interaction between Neuroligin/NLG-1 and DLG-1 or MAGI-1 in nematode Caenorhabditis elegans
复制标题
氧化石墨烯破坏线虫秀丽隐杆线虫中 Neuroligin/NLG-1 和 DLG-1 或 MAGI-1 之间的蛋白质-蛋白质相互作用
DOI：
10.1016/j.scitotenv.2019.134492
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发表时间：
2020-01-15
影响因子：
9.8
通讯作者：
Wang，Dayong
中科院分区：
文献类型：
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作者：
Zhao，Yunli;Chen，He;Wang，Dayong
氧化石墨烯(GO)是碳基工程纳米材料(ENM)。以秀丽线虫为动物模型，研究了GO暴露对蛋白质-蛋白质相互作用的影响。在线虫中，NLG-1/神经连接蛋白是一种突触后蛋白，只在神经元中发挥作用，调节GO的毒性。在神经元中，PSD-95蛋白DLG-1和S-SCAM蛋白MAGI-1被确定为NLG-1调节GO毒性的下游靶点。PKC-1是一种丝氨酸/苏氨酸蛋白激酶C，进一步作用于神经元DLG-1和MAGI-1的下游，调节GO的毒性。免疫共沉淀分析证实NLG-1与DLG-1或MAGI-1之间存在蛋白质相互作用。GO表达后，NLG-1与DLG-1或MAGI-1之间的这种蛋白质-蛋白质相互作用被显著抑制。因此，我们的数据提出了证据，表明GO暴露在破坏生物体中蛋白质-蛋白质相互作用的可能性。
Graphene oxide (GO) is a carbon-based engineered nanomaterial (ENM). UsingCaenorhabditis elegansas an animal model, we investigated the effect of GO exposure on protein-protein interactions. In nematodes, NLG-1/Neuroligin, a postsynaptic protein, acted only in the neurons to regulate the GO toxicity. In the neurons, DLG-1, a PSD-95 protein, and MAGI-1, a S-SCAM protein, were identified as the downstream targets of NLG-1 in the regulation of GO toxicity. PKC-1, a serine/threonine protein kinase C, further acted downstream of neuronal DLG-1 and MAGI-1 to regulate the GO toxicity. Co-immunoprecipitation analysis demonstrated the protein-protein interaction between NLG-1 and DLG-1 or MAGI-1. After GO expression, this protein-protein interaction between NLG-1 and DLG-1 or MAGI-1 was significantly inhibited. Therefore, our data raised the evidence to suggest the potential of GO exposure in disrupting protein-protein interactions in organisms.