Partial purification of Xenopus laevis egg extract factor(s) that induce swelling in permeabilized human sperm.
Partial purification of Xenopus laevis egg extract factor(s) that induce swelling in permeabilized human sperm.
复制标题
部分纯化非洲爪蟾卵提取因子，可诱导透化人类精子肿胀。
DOI：
10.1002/jez.1402580218
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Ruddle,FH
中科院分区：
文献类型：
--
作者：
Brown,DB;Miskimins,WK;Ruddle,FH
采用吸附（鱼精蛋白-琼脂糖）和凝胶过滤（Sephacryl S-300）色谱相结合的方法来富集非洲爪蟾卵提取物中诱导透化人类精子核膨胀-染色质解缩的因子。据确定，该纯化方案将诱导人类精子核肿胀的因子纯化了 70 倍。还原的活性因子的 Kavof 为 0.12，分子量约为 290,000 道尔顿。提取物核膨胀活性对50°C及以上的温度以及蛋白水解处理敏感。提取物的 RNase 和放线菌酮处理对核膨胀活性没有影响。这些数据表明，在透化的人类精子中诱导核膨胀的卵提取因子是存在于未受精青蛙卵中的蛋白质。
A combination of adsorption (protamine‐agarose) and gel filtration (Sephacryl S‐300) chromatography was used to enrich for factor(s) inXenopus laevisfrog egg extract that induce nuclear swelling‐chromatin decondensation in permeabilized human sperm. It was determined that a 70‐fold purification of the factor(s) that induce human sperm nuclear swelling has resulted from the purification scheme. The reduced, active factor(s) have a Kavof 0.12 and an approximate molecular weight of 290,000 daltons. The extract nuclear swelling activity is sensitive to temmperaturs of 50°C and above, as well as proteolytic treatment. RNase and cycloheximide treatments of the extracts have no effect on the nuclear swelling activity. These data suggest that the egg extract factor(s) that induce nuclear swelling in permeabilized human sperm are protein(s) that are present in the unfertilized frog egg.