Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation
Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation
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使用连接子介导的复杂策略和酵母遗传互补分析组蛋白变体 H2A.Z 的分子进化
DOI：
10.1093/bbb/zbab190
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Harata Masahiko
中科院分区：
文献类型：
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作者：
Kitagawa Saho;Kusakabe Masayuki;Takahashi Daisuke;Narimiya Takumi;Nakabayashi Yu;Seki Masayuki;Horigome Chihiro;Harata Masahiko
组蛋白变体H2A.Z通过特定的机制沉积到染色质中，并且是基因组功能所需的。使用接头介导的复杂的策略结合酵母遗传互补，我们证明了进化保守的H2A.Z连同其染色质掺入和功能。这种方法适用于与相互作用物形成复合物的蛋白质的进化分析。
The histone variant H2A.Z is deposited into chromatin by specific machinery and is required for genome functions. Using a linker-mediated complex strategy combined with yeast genetic complementation, we demonstrate evolutionary conservation of H2A.Z together with its chromatin incorporation and functions. This approach is applicable to the evolutionary analyses of proteins that form complexes with interactors.