miR-375 negatively regulates porcine preadipocyte differentiation by targeting BMPR2
miR-375 negatively regulates porcine preadipocyte differentiation by targeting BMPR2
复制标题
DOI：
10.1002/1873-3468.12169
复制
发表时间：
2016-05-01
期刊：
影响因子：
3.5
通讯作者：
Zhang, Jiabao
中科院分区：
文献类型：
--
作者：
Liu, Siyuan;Sun, Guangjie;Zhang, Jiabao
前脂肪细胞向脂肪组织的分化受到多种因素的严格调控，包括miRNAs和细胞因子。在本研究中，我们利用分离的猪原代前脂肪细胞，证明了miR-375的异位表达可以改变前脂肪细胞的分化。此外，骨形态发生蛋白受体2(BMPR2)被确定为miR-375的直接靶点。沉默BMPR2与过表达miR-375对前体脂肪细胞分化的抑制作用相同。总之，我们证明miR-375是猪原代前脂肪细胞成脂分化的负调控因子。这些结果阐明了miR-375在体外成脂分化中的作用。
The differentiation of preadipocytes into adipose tissues is tightly regulated by various factors including miRNAs and cytokines. In this study, taking advantage of isolated porcine primary preadipocytes, we showed that ectopic expression of miR-375 could change preadipocyte differentiation. In addition, bone morphogenetic protein receptor 2 (BMPR2) was identified as a direct target of miR-375. Silencing BMPR2 had the same inhibition effects as overexpressing miR-375 on the preadipocyte differentiation. Together, we demonstrated that miR-375 is a negative regulator of adipogenic differentiation using porcine primary preadipocytes. These results clarified the role of miR-375 in ex vivo adipogenic differentiation.