Rush Oral Immunotherapy Does Not Reduce Allergic Response in Mice with Mild Allergy to Egg White Ovomucoid
Rush Oral Immunotherapy Does Not Reduce Allergic Response in Mice with Mild Allergy to Egg White Ovomucoid
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DOI：
10.3177/jnsv.61.400
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发表时间：
2015-10-01
影响因子：
1.6
通讯作者：
Takahashi, Kyoko
中科院分区：
文献类型：
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作者：
Maeta, Akihiro;Kaji, Mayuko;Takahashi, Kyoko
口服免疫疗法（OTT）是治疗食物过敏的一种有前途的治疗方法。过去的研究表明，OTT仅在严重过敏模型小鼠中降低过敏反应。我们建立了轻度过敏模型小鼠，并研究了“匆忙”OTT 10 d是否改善了这些小鼠的过敏反应和生物标志物。Balb/c小鼠对明矾卵黏液（OM）致敏。快速OTT进行10 d。口服OM激发用于确定OTT对过敏反应的影响。我们测量了过敏生物标志物，如皮肤血管通透性、血浆总IgE、om特异性IgE、IgG1和IgG2a水平以及脾细胞培养上清中的细胞因子。OTT治疗10 d并没有改善过敏症状和增加血管通透性。经ott处理的小鼠血浆总IgE明显高于未处理的小鼠。ott处理和未处理小鼠的om特异性IgG1和IgG2a血浆水平无显著差异。油脂处理小鼠脾细胞分泌的细胞因子中，ifn - γ和IL-10显著低于未处理小鼠，IL-4和IL-5显著高于未处理小鼠。ot治疗组未检测总TGF-P。ott治疗组ifn - γ /IL-4比值约为未治疗组的1/8。在轻度过敏模型小鼠中，OTT治疗10 d无效，部分生物标志物出现阴性反应。我们建议OTT应该非常谨慎地使用，因为这种治疗有加重轻度过敏小鼠过敏症状的风险。
Oral immunotherapy (OTT) is a promising therapeutic approach for treating food allergy. Past studies have shown that OTT reduces allergic response only in severe allergy model mice. We worked to establish mild allergy model mice, and investigated whether 'rush' OTT for 10 d improved the allergic response and biomarkers in these mice. Balb/c mice were sensitized to ovomucoid (OM) in alum. The rush OTT was done for 10 d. Oral OM challenge was used to determine the impact of OTT on the allergic response. We measured allergic biomarkers, such as vascular permeability in the skin, plasma levels of total IgE, OM-specific IgE, IgG1 and IgG2a and cytokines in splenocyte culture supernatant. OTT for 10 d did not improve allergy symptoms and increased vascular permeability. Total IgE in the plasma of OTT-treated mice was significantly higher than in that of non-treated mice. OM-specific IgG1 and IgG2a plasma levels were not significantly different between OTT-treated and non-treated mice. Among the cytokine secretion of splenocyte from OIT-treated mice, IFN-gamma and IL-10 were significantly lower than in non-treated mice, and IL-4 and IL-5 were significantly higher. Total TGF-P in the OTT-treated group was not detected. The IFN-gamma/IL-4 ratio of the OTT-treated group was about 1/8 that of the non-treated group. OTT for 10 d was not effective and some biomarkers showed negative responses in the mild allergy model mice. We suggest OTT should be used very carefully as this treatment carries a risk of worsening allergy symptoms for mice with mild allergy.