Unacylated ghrelin, leptin, and appetite display diurnal rhythmicity in lean adults
Unacylated ghrelin, leptin, and appetite display diurnal rhythmicity in lean adults
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在瘦成年人中，未酰化的生长素释放肽、瘦素和食欲表现出昼夜节律性
DOI：
10.1152/japplphysiol.00920.2020
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发表时间：
2021
影响因子：
3.3
通讯作者：
Templeman I
中科院分区：
文献类型：
--
作者：
Templeman I
恒定的常规和强制性的去食欲方案通常去除行为/环境线索的影响以检查内源性昼夜节律，然而这可能不反映真实的世界中的食欲调节的节律。因此，重要的是要了解这些节奏在同一科目下控制昼夜条件下的光，睡眠和喂养。10名健康成人（9名男性/1名女性，平均值±标准差：年龄30 ± 10岁;体重指数24.1 ± 2.7 kg·m−2）在实验室仰卧休息37 h。所有数据都是在这段时间的最后24小时内收集的（即，0800-0800时）。参与者在2200至0700 h的睡眠机会之前的清醒期间每小时喂食等热量液体膳食替代品以及食欲评估。在24小时内每小时收集一次血液样本。昏暗的褪黑激素起效时间为2318 ± 46 min。确定了平均血浆非酰化生长激素释放肽浓度的昼夜节律（P= 0.04），在清醒后不久出现峰值（0819），在晚上降至最低点，相对振幅为9%。血浆瘦素浓度也呈现昼夜节律（P< 0.01），峰值出现在熄灯后（00 32 h），中午最低。该节律的振幅为25%。昼夜节律建立在所有方面的食欲，除了甜的偏好（P= 0.29），与饥饿（2103小时）和预期的食物消耗（1955小时）达到高峰，在晚上之前下降到他们的最低点后不久醒来。在控制昼夜条件下，同时测量瘦素，unacylated ghrelin，和主观食欲超过24小时的时间内显示节奏食欲调节在瘦，健康human.NEW和NOTEWORTHYSimultaneousassessment主观食欲，unacylated ghrelin，和瘦素进行了连续37小时的协议第一次在控制光，睡眠和喂养条件下的健康，瘦的成年人。在非酰化生长激素释放肽、瘦素和主观食欲的组成部分（如饥饿、预期消费和饱腹感）中观察到节律。同步测量这些变量的节律对于充分理解食欲组成部分之间的时间关系以及睡眠、光照和进食等昼夜因素的影响非常重要。
Constant routine and forced desynchrony protocols typically remove the effects of behavioral/environmental cues to examine endogenous circadian rhythms, yet this may not reflect rhythms of appetite regulation in the real world. It is therefore important to understand these rhythms within the same subjects under controlled diurnal conditions of light, sleep, and feeding. Ten healthy adults (9 M/1 F, means ±SD: age, 30 ± 10 yr; body mass index, 24.1 ± 2.7 kg·m−2) rested supine in the laboratory for 37 h. All data were collected during the final 24 h of this period (i.e., 0800–0800 h). Participants were fed hourly isocaloric liquid meal replacements alongside appetite assessments during waking before a sleep opportunity from 2200 to 0700 h. Hourly blood samples were collected throughout the 24-h period. Dim light melatonin onset occurred at 2318 ± 46 min. A diurnal rhythm in mean plasma unacylated ghrelin concentration was identified (P= 0.04), with the acrophase occurring shortly after waking (0819), falling to a nadir in the evening with a relative amplitude of 9%. Plasma leptin concentration also exhibited a diurnal rhythm (P< 0.01), with the acrophase occurring shortly after lights-out (0032 h) and the lowest concentrations at midday. The amplitude for this rhythm was 25%. Diurnal rhythms were established in all dimensions of appetite except for sweet preference (P= 0.29), with both hunger (2103 h) and prospective food consumption (1955 h) reaching their peak in the evening before falling to their nadir shortly after waking. Under controlled diurnal conditions, simultaneous measurement of leptin, unacylated ghrelin, and subjective appetite over a 24-h period revealed rhythmicity in appetite regulation in lean, healthy humans.NEW & NOTEWORTHYSimultaneous assessment of subjective appetite, unacylated ghrelin, and leptin was carried out over a continuous 37-h protocol for the first time under conditions of controlled light, sleep, and feeding in healthy, lean adults. Rhythms were observed in unacylated ghrelin, leptin, and components of subjective appetite, such as hunger, prospective consumption, and fullness. Concurrent measurement of rhythms in these variables is important to fully understand the temporal relationships between components of appetite as well as the influence of diurnal factors such as sleep, light, and feeding.