New New cytotoxic ergostane-type sterols from the Chinese soft coral Sinularia sp.
New New cytotoxic ergostane-type sterols from the Chinese soft coral Sinularia sp.
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DOI：
10.1016/j.steroids.2019.108425
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发表时间：
2019-09-01
期刊：
影响因子：
2.7
通讯作者：
Guo, Yue-Wei
中科院分区：
文献类型：
--
作者：
Jiang, Cheng-Shi;Ru, Tong;Guo, Yue-Wei
从软珊瑚Sinularia sp中分离到两个新的高氧麦角甾类甾醇（1,2）和一个已知的相关化合物sinugransterol A(3)。通过详细的光谱分析、化学转化和与文献报道的比较，对它们的结构进行了鉴定。在生物测定中，化合物1和2显示出对一系列癌细胞系的体外抗增殖活性，包括MDA-MB-436、A549、Hep3B、HT-29和H157。形态学观察和Hoechst 33,258染色表明，化合物1处理的H157细胞具有凋亡特征。此外，western blot检测显示，1可以上调Bax的表达，下调Bcl-2的表达。
Two new highly oxygenated ergostane-type sterols (1, 2) together with one known related compound sinugrandisterol A (3) were isolated from the soft coral Sinularia sp. collected from the water area near the Xisha Islands, South China Sea. Their structures were elucidated on the basis of detailed spectroscopic analysis, chemical transformations, and by comparison with those reported in literature. In bioassays, compounds 1 and 2 showed in vitro antiproliferative activity against a panel of cancer cell lines, including MDA-MB-436, A549, Hep3B, HT-29 and H157. Morphological observation and Hoechst 33,258 staining assays showed that compound 1-treated H157 cells displayed apoptosis characteristics. Moreover, western blot assays suggested that 1 could increase the expression of Bax and down-regulate expression of Bcl-2.