Upregulated expression of ACTL8 contributes to invasion and metastasis and indicates poor prognosis in colorectal cancer
Upregulated expression of ACTL8 contributes to invasion and metastasis and indicates poor prognosis in colorectal cancer
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ACTL8表达上调有助于结直肠癌的侵袭和转移并提示不良预后
DOI：
10.2147/ott.s185858
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发表时间：
2019-01-01
影响因子：
4
通讯作者：
Wei, Min-Jie
中科院分区：
文献类型：
--
作者：
Han, Qiang;Sun, Ming-Li;Wei, Min-Jie
背景：ACTL8是CT抗原的一个成员。关于ACTL8在恶性肿瘤中的作用的研究很少。本研究旨在探讨ACTL8蛋白在结直肠癌组织中的表达及其临床意义。材料与方法：收集人结直肠癌组织、结直肠癌细胞株、癌旁组织及人肠上皮细胞株，检测ACTL8蛋白的表达。研究ACTL8蛋白表达与结直肠癌患者临床病理参数和预后之间的关系。在CRC细胞系中ACTL8沉默后，通过伤口愈合和transwell侵袭测定来确定ACTL8在CRC侵袭和转移中的生物学功能。结果：ACTL8在人大肠癌组织和大肠癌细胞系中表达上调。ACTL8的表达与肿瘤的分化程度、浸润转移、术后感染、预后不良呈正相关，与近端切缘长度呈负相关。此外，ACTL8的沉默显著降低了HT 29和SW 620 CRC细胞系的侵袭和迁移能力。结论：ACTL8在大肠癌的侵袭和转移中起重要作用，TRIM29可能参与了ACTL8介导的大肠癌不良预后。
Background: ACTL8 is a member of the CT antigens. There are only few studies on the role of ACTL8 in malignant tumors. The aim of this study is to investigate the expression and clinical significance of ACTL8 protein in colorectal cancer (CRC).Materials and methods: Human CRC tissues and cell lines, and paired adjacent non-tumor tissues and human intestinal epithelial cell lines were obtained to evaluate the expression of ACTL8. The association between protein expression of ACTL8 and clinicopathological parameters and prognosis of CRC patients was examined. The biological functions of ACTL8 in the invasion and metastasis of CRC were determined by wound healing and transwell invasion assays after silencing of ACTL8 in CRC cell lines. The potential target genes of ACTL8 were also identified by quantitative reverse transcription PCR and Western blotting after silencing of ACTL8 in CRC cell lines.Results: It was found that ACTL8 was upregulated in human CRC tissues and cell lines. The expression of ACTL8 was positively associated with poor differentiation, invasion and metastasis, postoperative infection, and poor prognosis, but negatively associated with proximal margin length. In addition, silencing of ACTL8 significantly decreased the capacity of invasion and migration in HT29 and SW620 CRC cell lines. Moreover, silencing of ACTL8 significantly decreased the expression of TRIM29 in HT29 and SW620 CRC cell lines.Conclusion: These results suggest that ACTL8 plays a key role in the invasion and metastasis of CRC, and TRIM29 may be involved in the ACTL8-mediated poor prognosis of CRC.