Gas-phase removal of biofilms from various surfaces using carbon dioxide aerosols
Gas-phase removal of biofilms from various surfaces using carbon dioxide aerosols
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DOI：
10.1080/08927014.2012.701624
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发表时间：
2012-01-01
期刊：
影响因子：
2.7
通讯作者：
Jang, Jaesung
中科院分区：
文献类型：
--
作者：
Cha, Minju;Hong, Seongkyeol;Jang, Jaesung
本研究评估了去除大肠杆菌XL 1-蓝色生物膜使用定期射流的二氧化碳气溶胶（固体和气体CO2的混合物）与氮气。气溶胶是由高压CO2气体通过喷嘴绝热膨胀产生的，并用于去除空气干燥的生物膜。在施用气溶胶之前和之后，从扫描电子显微照片测量生物膜的面积。气溶胶处理的去除效率的测量与各种空气干燥时间的生物膜处理前，表面材料，和持续时间的CO2气溶胶在每个8秒的气溶胶-氮气清洁周期。在90 s内，几乎100%的新鲜生物膜从各种表面非常可靠地去除。该技术可用于去除固体表面上的不饱和生物膜，并具有清洁生物污染表面的潜在应用。
The present study evaluated the removal of Escherichia coli XL1-blue biofilms using periodic jets of carbon dioxide aerosols (a mixture of solid and gaseous CO2) with nitrogen gas. The aerosols were generated by the adiabatic expansion of high-pressure CO2 gas through a nozzle and used to remove air-dried biofilms. The areas of the biofilms were measured from scanning electron micrographs before and after applying the aerosols. The removal efficiency of the aerosol treatment was measured with various air-drying times of the biofilms before the treatment, surface materials, and durations of CO2 aerosols in each 8-s aerosol-nitrogen cleaning cycle. Nearly 100% of the fresh biofilms were removed from the various surfaces very reliably within 90 s. This technique can be useful for removing unsaturated biofilms on solid surfaces and has potential applications for cleaning bio-contaminated surfaces.