Toxicity Evaluation of Carbon Nanotubes in Normal Human Bronchial Epithelial Cells
Toxicity Evaluation of Carbon Nanotubes in Normal Human Bronchial Epithelial Cells
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DOI：
10.1166/jbn.2011.1225
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发表时间：
2011-02-01
影响因子：
2.9
通讯作者：
Pandey, Alok K.
中科院分区：
文献类型：
--
作者：
Mittal, Sandeep;Sharma, Vyom;Pandey, Alok K.
碳纳米管（CNT）以其优异的热、机械和电学性能而闻名。对于许多碳纳米管应用来说，最重要的是了解与接触有关的健康危害。正常人支气管上皮细胞（BEAS-2B）已用于评估SWCNT（直径-1.2-1.5 nm）和DWCNT（直径-1.3-5 nm）的细胞毒性。发现CNT与常规体外细胞毒性测定（MU、NRU和LDH）染料的明显干扰，这通过无细胞系统证实。细胞形态学改变和流式细胞仪检测显示细胞毒性特征。因此，我们的研究表明，有必要适当的方法来评估碳纳米管的细胞毒性。
Carbon nanotubes (CNTs) are well known for their exceptional thermal, mechanical and electrical properties. For many CNT applications it is of the foremost importance to know their health hazards related to their exposure. Normal human bronchial epithelial cells (BEAS-2B) has been used for assessment the cytotoxicity of SWCNT (Diameter-1.2-1.5 nm) and DWCNT (Diameter-1.3-5 nm). Clear interference of CNTs with conventional in vitro cytotoxicity assays (MU, NRU and LDH) dye was found which was confirmed by acellular system. However morphological changes and flow cytometry showed the characteristics of cytotoxicity. Thus our study showed that there is a need of appropriate method for the assessment of cytotoxicity of CNT.