Sensitive Fluorescence Detection of Etimicin Based on Derivatives of Formaldehyde and Acetylacetone
Sensitive Fluorescence Detection of Etimicin Based on Derivatives of Formaldehyde and Acetylacetone
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DOI：
10.1007/s10895-012-1121-6
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发表时间：
2013-01-01
影响因子：
2.7
通讯作者：
Cao, Lu
中科院分区：
文献类型：
--
作者：
Zhuang, Yafeng;Cao, Lu
A novel fluorescence method for the determination of etimicin is described. Etimicin reacts with acetylacetone and formaldehyde in pH 4.0 Britton-Robinson (B.R.) buffer solution to from a fluorescent substance [I]. Emission spectra of [I] and the reagent blank were overlapped, so the arithmetic emission spectra of the fluorescent substance were obtained by subtracted form the spectra of [I] to the spectra of the reagent blank using the Fluorescence Data Software. There is a linear relationship between the intensity of the arithmetic emission spectra and the concentration of etimicin. Effects of pH, amount of acetylacetone-formaldehyde, and heating time on the determination of etimicin have been examined. Etimicin can be determined over the concentration range of 1.0 to 10.0 mu g mL(-1) with a correlation coefficient of 0.9991. The relative standard deviation (RSD) for 11 repetitive determinations of 5.0 mu g mL(-1) etimicin is 0.22 %. The utility of this method was demonstrated by determining etimicin in commercial samples.
