Identification of a target for CudA, the transcription factor which directs formation of the Dictyostelium tip organiser
Identification of a target for CudA, the transcription factor which directs formation of the Dictyostelium tip organiser
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DOI：
10.1387/ijdb.082723hw
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发表时间：
2010-01-01
影响因子：
0.7
通讯作者：
Williams, Jeffrey G.
中科院分区：
文献类型：
--
作者：
Wang, Hong-Yu;Williams, Jeffrey G.
网骨藻蛞蝓的顶端的功能很像胚胎组织器;当移植到受体蛞蝓的侧面时，它会招募大量的前孢子细胞，并将它们作为次级蛞蝓的一部分带走。CudA是在前孢子细胞中表达的核蛋白，在那里它作为特异性转录因子。CudA也在位于前部的细胞群中表达，即被认为构成功能性尖端的尖端组织者。我们确定了一个扩展蛋白样基因，exp 17，这是在提示组织者区域内表达，这是不表达的cudA空菌株。exp 17启动子含有一个区域，在体外结合到CudA，这个区域是必要的，在尖端组织者的表达。这些结果为先前定义的信号转导途径提供了终点，其中ACA腺苷酸环化酶在尖端组织者内的区域化表达导致局部cAMP诱导的STATa活化和随后STATa与cudA启动子的结合。然后，STATa诱导cudA的表达，cudA指导靶基因如exp 17的转录。
The tip of the Dictyostelium slug functions much like an embryonic organiser; when grafted onto the flank of a recipient slug, it recruits amass of prespore cells and leads them away as part of a secondary slug. CudA is a nuclear protein which is expressed in prespore cells where it acts as a specific transcription factor. CudA is also expressed in an anteriorly located group of cells, the tip-organiser, that is believed to constitute the functional tip. We identify an expansin-like gene, exp17, that is expressed within the tip-organiser region and which is not expressed in a cudA null strain. The exp17 promoter contains a region that binds to CudA in vitro and this region is necessary for expression in the tip-organiser. These results provide an end-point for a previously defined signal transduction pathway in which regionalised expression of the ACA adenylyl cyclase within the tip-organiser leads to localised cAMP-induced activation of STATa and consequent binding of STATa to the cudA promoter. STATa then induces expression of cudA and cudA directs the transcription of target genes such as exp17.