Fluorescein derivatization of fibrinogen for flow cytometric analysis of fibrinogen binding to platelets.
Fluorescein derivatization of fibrinogen for flow cytometric analysis of fibrinogen binding to platelets.
复制标题
纤维蛋白原的荧光素衍生化，用于纤维蛋白原与血小板结合的流式细胞术分析。
DOI：
10.1002/cyto.990170403
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Dale,GL
中科院分区：
文献类型：
--
作者：
Heilmann,E;Hynes,LA;Burstein,SA;George,JN;Dale,GL
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Dog and human fibrinogen were derivatized with N‐hydroxysuccinimidofluorescein and utilized for flow cytometric estimation of fibrinogen binding to activated platelets. Fluorescein‐fibrinogen binding fulfilled the criteria for specific binding to platelets; the binding was saturable, dependent on agonist activation, and inhibited by unlabeled fibrinogen. In addition, EDTA and barbourin, a KGD‐containing peptide, were found to inhibit the binding of fluorescein‐fibrinogen. Fluorescein‐fibrinogen bound to dog platelets with an apparent affinity of 0.31 μM after stimulation with either adenosine‐5′‐diphosphate (ADP) or plateletactivating factor. The labeled fibrinogen was also used to study the fibrinogen binding capacity of aged, biotinylated platelets. Aged platelets were indistinguishable from young platelets with regard to fibrinogen binding in response to ADP. These studies document that direct derivatization of fibrinogen with fluorescein generates a useful probe for analyzing fibrinogen binding to platelets with flow cytometry. © 1994 Wiley‐Liss, Inc.