Differentiation of human neural stem cells into retinal cells
Differentiation of human neural stem cells into retinal cells
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DOI：
10.1097/00001756-200301200-00026
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发表时间：
2003-01-20
期刊：
影响因子：
1.7
通讯作者：
Sugaya, K
中科院分区：
文献类型：
--
作者：
Dong, X;Pulido, JS;Sugaya, K
我们以前曾报道，移植的人神经干细胞（HNSCs）显示出广泛的迁移和位置纳入老年大鼠的大脑，这是与认知功能的改善。在当前的研究中，为了研究HNSCs是否能够分化为视网膜细胞，我们在无血清分化条件下用人转化生长因子-β 3（TGF-β 3）处理HNSCs。在体外分化5天后，我们在用TGF-β 3处理的培养物中检测到视蛋白免疫阳性细胞。我们还将TGF-β 3处理的HNSCs移植到大鼠玻璃体腔中。供体细胞在宿主视网膜细胞层中迁移并分化为视蛋白阳性细胞。在这里，我们第一次表明，TGF-β 3处理的HNSCs分化成视网膜细胞。
We have previously reported that transplanted human neural stem cells (HNSCs) display extensive migration and positional incorporation into the aged rat brain, which is associated with an improvement in cognitive function. In the current study, to investigate whether HNSCs are capable of differentiating into retinal cells, we treated HNSCs with human transforming growth factor-beta3 (TGF-beta3) under a serum-free differentiation condition. After 5 days of differentiation in vitro we detected opsin-immunopositive cells in the culture treated with TGF-beta3. We also transplanted TGF-beta3-treated HNSCs into the rat vitreous cavity. The donor cells migrated and differentiated into opsin-positive cells in the host retinal cell layer. Here we show for the first time that TGF-beta3-treated HNSCs differentiate into retinal cells.