Biochemical Aspects of the Coagulation and Liquefaction of Human Semen
Biochemical Aspects of the Coagulation and Liquefaction of Human Semen
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人类精液凝固和液化的生化方面
DOI：
10.1002/j.1939-4640.1980.tb00043.x
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发表时间：
1980
影响因子：
--
通讯作者：
L. J. Zaneveld
中科院分区：
文献类型：
--
作者：
P. F. Tauber;D. Propping;G. Schumacher;L. J. Zaneveld
对人精液的凝固液化过程进行了较详细的研究。结果发现，精囊液与其他附属性腺分泌物或精子的接触似乎不是凝结物形成的必要条件，肝素和柠檬酸钠不影响凝结物的形成，表明精液凝结过程与血块凝结过程之间的差异。关于精液凝固物的液化过程，结果表明：（1）精子不影响液化过程，（2）精浆纤溶酶原激活物、溶菌酶、α-淀粉酶、胃蛋白酶和神经氨酸酶不是液化的主要因素; 3）所述增稠剂存在于分开的射精的第一部分中，是热不稳定的，在-20 ℃冷冻时部分破坏，但在冻干时稳定，可与（NH 4）2SO 4沉淀，不受血清或EDTA影响，可被兔胆汁抑制，但不被精浆蛋白酶抑制剂、Kunitz胰蛋白酶抑制剂或ε-氨基己酸（EACA）抑制。所有这些特性都是精液酶-这种酶的部分纯化制剂增强了液化作用，两名低活性的患者溶解凝固物的能力很差。这些发现证实了精浆蛋白是引起精液凝固物液化的主要因素，进一步表明精液凝固物液化的机制与血块溶解的机制不同。
The coagulation and liquefaction process of human semen was studied in some detail. It was found that contact of seminal vesicle fluid with the other accessory sex gland secretions or spermatozoa does not seem to be essential for the formation of the coagulum, and that heparin and sodium citrate do not affect coagulum formation, indicating a difference between the seminal coagulation process and that of the blood clot. Regarding the liquefaction process of the seminal coagulum, it was shown that 1) spermatozoa do not influence liquefaction; 2) that the seminal plasminogen activator, lysozyme, α-amylase, pepsin, and neuramini dase are not the primary liquefying factors; 3) that the liquefying agent is present in the first portion of a split ejaculate, is heat labile, is partially destroyed by freezing at −20 C but is stable to lyophilization, is precipitable with (NH4)2SO4, is not affected by serum or EDTA, and is inhibited by rabbit bile but not by the seminal plasma proteinase inhibitors, the Kunitz pancreatic trypsin inhibitor, or epsilon-aminocaproic acid (EACA). All of these prop erties are characteristics of the seminal en zyme, seminin. A partially purified preparation of this enzyme enhanced liquefaction, and two patients who had low seminin activity pos sessed poorly lysing coagula. These findings confirm that seminin is the agent primarily re sponsible for liquefaction of the seminal coagulum of man. The data further show that liquefaction of the seminal coagulum occurs by a different mechanism than that of the lysis of the blood clot.