Analysis of the In Vivo Function of Follicular Regulatory T (TFR) Cells in the Regulation of Antibody Response.
Analysis of the In Vivo Function of Follicular Regulatory T (TFR) Cells in the Regulation of Antibody Response.
复制标题
滤泡调节 T (TFR) 细胞在抗体反应调节中的体内功能分析。
DOI：
10.1007/978-1-0716-2647-4_1
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Leavenworth,JianmeiW
中科院分区：
文献类型：
--
作者：
Luo,Lin;Leavenworth,JianmeiW
Follicular regulatory T (TFR) cells, a subset of Foxp3+regulatory T cells (Tregs), play an essential role in the regulation of germinal center (GC) response and humoral self-tolerance. Although it is generally accepted that TFRcells suppress GC antibody responses mediated by follicular helper T (TFH) cells and B cells, the helper activity of TFRcells on GC responses has also been recently reported. Because of this, it is critical to develop specific assays that are able to precisely assess TFRcell function, particularly its in vivo activity, independent of differentiation and other Tregs. Here we describe an adoptive transfer approach in conjugation with flow cytometry and ELISA to evaluate the TFRcell function on TFH, B cells, and antibody response in vivo.
