Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers
Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers
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DOI：
10.1023/a:1020652215048
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发表时间：
2002-11-01
影响因子：
9.1
通讯作者：
Gibson, RA
中科院分区：
文献类型：
--
作者：
Hawkes, JS;Bryan, DL;Gibson, RA
于产后5周采集82名哺乳期母亲的乳汁和静脉血样本(n=80)。分离人乳细胞和外周血单个核细胞，用酶联免疫吸附试验检测在无脂多糖和有无脂多糖存在的情况下，人乳细胞产生IL-1β、IL-6和肿瘤坏死因子-α。在没有刺激的情况下，人乳细胞自发产生的白介素1β、白介素6和肿瘤坏死因子-α显著低于外周血单核细胞。在体外，人乳细胞在脂多糖(500 ng/ml)刺激24小时后，细胞因子的产生增加了约40-50%，而外周血单个核细胞对脂多糖(200 ng/ml)的反应，细胞因子的产生增加了350%。这些观察表明，牛奶中的细胞能够积极参与乳腺中白细胞介素1β、白细胞介素6和肿瘤坏死因子α的产生，并有能力在离开乳房后对进一步的刺激做出反应。
Samples of milk (n = 80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40-50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in the mammary gland and have the capacity to respond to further stimulation after leaving the breast.