Lesion recovery and the bacterial microbiome in two Caribbean gorgonian corals
Lesion recovery and the bacterial microbiome in two Caribbean gorgonian corals
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DOI：
10.1007/s00227-016-3008-6
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发表时间：
2016-10
期刊：
影响因子：
2.4
通讯作者：
K. P. Shirur;C. Jackson;T. Goulet
中科院分区：
文献类型：
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作者：
K. P. Shirur;C. Jackson;T. Goulet
在加勒比地区，柳珊瑚在许多珊瑚礁中占主导地位，而石珊瑚的覆盖率则有所下降。柳珊瑚处理压力源，如由波浪作用，捕食或人类活动引起的病变。2012年6月，在墨西哥莫雷洛斯港附近的一块礁（20°52′5.23″N，86°51′58.92″W）上，在3-5米深处发现了柳珊瑚Eunicea flexuosa和Pseudoplexaura porosa的分支上造成了人工伤口。愈合后，收集受伤和未受伤的分支，以确定病变对柳珊瑚生物化学，共生，微生物组和免疫反应的影响。E.病变恢复。flexuosatook的长度是inP的两倍。多孔的。在这两个物种中，组织和周围的病变含有显着较高的骨片含量每干重，但较低的蛋白质每表面积。在病变区域及其周围，共生甲藻，共生藻属，虽然病斑周围组织中共生菌的光化学效率不受影响，但其光化学效率低于未受损伤的枝条。柳珊瑚物种的细菌微生物组不同，但受伤和未受伤树枝之间的总体细菌群落和优势细菌分类群没有差异，尽管一些不太常见的细菌群的流行率确实有所不同。这两个物种表现出不同的免疫反应，从而黑化级联的不同组件被激活，和外几丁质酶被动员，只有在大肠杆菌。弯曲的。虽然柳珊瑚物种的病变恢复反应不同，但两者都愈合了，没有疾病或污损生物定植的迹象。从受伤中成功恢复的能力可能部分解释了为什么柳珊瑚在加勒比海珊瑚礁中占主导地位。
In the Caribbean, gorgonian corals dominate many coral reefs, while scleractinian coral cover has declined. Gorgonian corals deal with stressors such as lesions caused by wave action, predation, or human activities. In June 2012, artificial wounds were inflicted on branches of the gorgoniansEunicea flexuosaandPseudoplexaura porosafound at 3–5 m depth on a patch reef (20°52′5.23″N, 86°51′58.92″W) near Puerto Morelos, Mexico. Following healing, injured and uninjured branches were collected to determine the effect of lesions on gorgonian biochemistry, symbiosis, microbiome, and immune response. Lesion recovery inE. flexuosatook twice as long as inP. porosa. In both species, tissues at and surrounding the lesions contained significantly higher sclerite content per dry weight but lower protein per surface area. In and around the lesion area, the density of symbiotic dinoflagellates,Symbiodiniumspp., was lower than in uninjured branches, althoughSymbiodiniumphotochemical efficiency in tissues surrounding the lesion was not affected. The gorgonian species differed in their bacterial microbiome, but the overall bacterial community and dominant bacterial taxa did not differ between injured and uninjured branches, although the prevalence of some less common bacterial groups did vary. The two species exhibited distinct immune responses, whereby different components of the melanization cascade were activated, and exochitinase was mobilized only inE. flexuosa. While the gorgonian species differed in their lesion recovery response, both healed without signs of disease or colonization by fouling organisms. The capacity to recover successfully from injuries may partly explain why gorgonian corals dominate Caribbean coral reefs.