Spalt-mediated dve repression is a critical regulatory motif and coordinates with Iroquois complex in Drosophila vein formation
Spalt-mediated dve repression is a critical regulatory motif and coordinates with Iroquois complex in Drosophila vein formation
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Spalt 介导的 dve 抑制是一个关键的调节基序，并与果蝇静脉形成中的易洛魁复合体协调
DOI：
10.1016/j.mod.2016.06.004
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发表时间：
2016
影响因子：
2.6
通讯作者：
and Hideki Nakagoshi
中科院分区：
文献类型：
--
作者：
Seiko Sugimori;Aya Hasegawa;and Hideki Nakagoshi
静脉是维持翅膀形状的纵向角质结构。黑腹果蝇有六个纵向静脉 (L1–L6) 和两个交叉静脉。 Spalt-complex (Sal) 的 Zn-finger 转录因子是 L2 和 L5 定位所必需的，Iroquois 复合体 (Iro-C) 的同源域转录因子是 L3 和 L5 静脉形成所必需的。同源域转录抑制子缺陷腺胃 (Dve) 在幼虫成虫盘的翼囊中均匀表达。然而，运动突变的翅膀显示 L2 和 L5 静脉缺失，但 L3 和 L4 静脉没有缺失。 Temporaldveknockdown 实验表明，从第三幼虫龄晚期到预蛹阶段，Dve 活性是静脉形成所必需的。在蛹前翅中，通过 Sal 介导的 dve 抑制，Dve 表达几乎与 Sal 的表达互补。此外，Dve 和 Iro-C 的共表达解除了 Sal 介导的抑制，是 L5 形成以剂量依赖性方式所必需的。翼静脉规格中 Sal、Dve 和 Iro-C 之间的关系与小眼细胞类型规格中的关系非常相似。我们的结果提供了有关细胞分化中调节基序的保守功能的信息。
Veins are longitudinal cuticular structures that maintain shape of the wing.Drosophila melanogasterhas six longitudinal veins (L1–L6) and two cross veins. The Zn-finger transcription factors of Spalt-complex (Sal) are required for positioning of the L2 and L5, and the homeodomain transcription factors of Iroquois complex (Iro-C) are required for formation of the L3 and L5 veins.The homeodomain transcriptional repressor Defective proventriculus (Dve) is uniformly expressed in the wing pouch of the larval imaginal disc. However,dvemutant wings showed loss of the L2 and L5, but not of the L3 and L4 veins. Temporaldveknockdown experiments indicate that the Dve activity is required for vein formation from late third larval instar to the prepupal stage. In the prepupal wing, Dve expression becomes nearly complementary to that of Sal through the Sal-mediateddverepression. Furthermore, coexpression of Dve and Iro-C relieved of Sal-mediated repression is required for the L5 formation in a dose-dependent manner. The relationship between Sal, Dve, and Iro-C in wing vein specification is quite similar to that in ommatidial cell-type specification. Our results provide information about the conserved function ofdveregulatory motifs in cell differentiation.