alpha2-Adrenergic receptor-mediated modulation of calcium current in neocortical pyramidal neurons.
alpha2-Adrenergic receptor-mediated modulation of calcium current in neocortical pyramidal neurons.
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α2-肾上腺素受体介导的新皮质锥体神经元钙电流调节。
DOI：
10.1016/j.brainres.2004.04.025
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Foehring,RC
中科院分区：
文献类型：
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作者：
Timmons,SD;Geisert,E;Stewart,AE;Lorenzon,NM;Foehring,RC
去甲肾上腺素能投射到皮质调节各种皮质活动，钙通道是这种调节的一个可能的靶点。我们用全细胞膜片钳技术研究了去甲肾上腺素能对大鼠感觉运动皮层锥体神经元钡电流的调制。细胞外应用特异性激动剂和拮抗剂发现去甲肾上腺素（NE）可降低Ca ~（2+）电流。这种调节的主要成分是由于α 2受体的激活。α2-肾上腺素能受体的激活导致涉及Gi/GoG蛋白的快速电压依赖性途径。该通路靶向N型和P型钙通道 重复动作电位波形（APW）可部分逆转α 2调制。N-和P-型通道已被牵连在这些细胞的突触传递和激活后超极化。我们的研究结果表明，NE可以调节这些细胞过程的机制敏感的尖峰活动。
Noradrenergic projections to the cortex modulate a variety of cortical activities and calcium channels are one likely target for such modulation. We used the whole-cell patch-clamp technique to study noradrenergic modulation of barium currents in acutely dissociated pyramidal neurons from rat sensorimotor cortex. Extracellular application of specific agonists and antagonists revealed that norepinephrine (NE) reduced Ca2+current. A major component of this modulation was due to activation of α2receptors. Activation of α2-adrenergic receptors resulted in a fast, voltage-dependent pathway involving Gi/GoG-proteins. This pathway targeted N- and P-type calcium channels The α2modulation was partially reversed by repeated action potential waveforms (APWs). N- and P-type channels have been implicated in synaptic transmission and activation of afterhyperpolarizations in these cells. Our findings suggest that NE can regulate these cellular processes by mechanisms sensitive to spike activity.