Identification of a functional allene oxide synthase-lipoxygenase fusion protein in the soft coral Gersemia fruticosa suggests the generality of this pathway in octocorals
Identification of a functional allene oxide synthase-lipoxygenase fusion protein in the soft coral Gersemia fruticosa suggests the generality of this pathway in octocorals
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DOI：
10.1016/j.bbagen.2007.10.010
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发表时间：
2008-02-01
影响因子：
3
通讯作者：
Samel, Nigulas
中科院分区：
文献类型：
--
作者：
Lohelaid, Helike;Jarving, Reet;Samel, Nigulas
在植物中，脂肪酸氢过氧化物转化为丙二烯环氧化物由细胞色素P450催化。与此相反，在珊瑚Plexaura homomalla，过氧化氢酶相关的血红素蛋白融合的脂氧合酶（LOX）被发现作为丙二烯氧化物合酶的功能。本工作报告了基于同源性的RT-PCR克隆和功能表达的Gersemia fruticosa类似物的丙二烯氧化物脱氢酶-脂氧合酶（AOS-LOX）融合蛋白。共和党fruticosa mRNA编码与P. homomalla AOS-LOX具有84%序列同一性的蛋白质。我们的数据表明，AOS-LOX融合蛋白途径是由另一种珊瑚和P homomalla代表没有例外。(c)2007 Elsevier B. V.保留所有权利。
The conversion of fatty acid hydroperoxides to allene epoxides is catalysed by a cytochrome P450 in plants. In contrast, in the coral Plexaura homomalla, a catalase-related hemoprotein fused to the lipoxygenase (LOX) was found to function as an allene oxide synthase. This work reports the homology-based RT-PCR cloning and functional expression of a Gersemia fruticosa analogue of the allene oxide synthase-lipoxygenase (AOS-LOX) fusion protein. The G. fruticosa mRNA codes for a protein with 84% sequence identity to the P. homomalla AOS-LOX. Our data indicate that the AOS-LOX fusion protein pathway is used by another coral and P homomalla represents no exception. (c) 2007 Elsevier B.V. All rights reserved.