A multi-object spectral imaging instrument
A multi-object spectral imaging instrument
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DOI：
10.1088/2040-8978/15/8/085302
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发表时间：
2013-08-01
影响因子：
2.1
通讯作者：
Padgett, M. J.
中科院分区：
文献类型：
--
作者：
Gibson, G. M.;Dienerowitz, M.;Padgett, M. J.
我们开发了一种快照光谱成像系统，可安装在商用倒置显微镜的侧面摄像头端口上。该系统实时提供显微镜图像上随机选择的多个点的光谱。使用数字微镜装置将样品中选定点的光从侧端口成像臂引导至基于色散棱镜和 CCD 相机的光谱仪臂。多线激光源用于校准 CCD 上像素位置的波长。显微镜前端口上的 CMOS 摄像头可以显示样品的完整图像，也可用于粒子跟踪，提供样品中移动的多个粒子的光谱。我们通过记录水溶液中多个荧光珠的光谱以及沿着含有红色和蓝色染料混合物的显微镜样品通道的多个点来演示该系统。
We have developed a snapshot spectral imaging system which fits onto the side camera port of a commercial inverted microscope. The system provides spectra, in real time, from multiple points randomly selected on the microscope image. Light from the selected points in the sample is directed from the side port imaging arm using a digital micromirror device to a spectrometer arm based on a dispersing prism and CCD camera. A multi-line laser source is used to calibrate the pixel positions on the CCD for wavelength. A CMOS camera on the front port of the microscope allows the full image of the sample to be displayed and can also be used for particle tracking, providing spectra of multiple particles moving in the sample. We demonstrate the system by recording the spectra of multiple fluorescent beads in aqueous solution and from multiple points along a microscope sample channel containing a mixture of red and blue dye.