LIMITED DIVERSITY OF T-CELL RECEPTOR GAMMA-CHAIN EXPRESSION OF MURINE THY-1+ DENDRITIC EPIDERMAL-CELLS REVEALED BY V-GAMMA-3-SPECIFIC MONOCLONAL-ANTIBODY
LIMITED DIVERSITY OF T-CELL RECEPTOR GAMMA-CHAIN EXPRESSION OF MURINE THY-1+ DENDRITIC EPIDERMAL-CELLS REVEALED BY V-GAMMA-3-SPECIFIC MONOCLONAL-ANTIBODY
复制标题
DOI：
10.1073/pnas.86.11.4185
复制
发表时间：
1989-06-01
影响因子：
11.1
通讯作者：
ALLISON, JP
中科院分区：
文献类型：
--
作者：
HAVRAN, WL;GRELL, S;ALLISON, JP
为了研究小鼠中Thy-1+树突状表皮细胞（Thy-1+德克斯）的T细胞抗原受体（TCR）多样性的起源和程度，我们开发了针对γ的单克隆抗体（mAb 536）。δ的TCR。mAb 536结合并刺激Thy-1+ dEC分泌白细胞介素2，但不结合并刺激表达由α组成的TCR的细胞分泌白细胞介素2。和β店mAb 536沉淀CD 3相关的γ。和δ来自放射性碘标记的Thy-1+德克斯的裂解物的链。一组表达γ的杂交瘤的分析δ的TCR表明mAb 536限定了可变区（V γ 3）基因产物的表位。流式细胞术分析显示，成年小鼠中V γ 3的表达限于表皮中的细胞，其中基本上所有Thy-1+细胞都是V γ 3+。14天胎儿胸腺中的大多数CD 3+细胞也表达V γ 3。这些结果表明，表皮中的T细胞补体是表达γ的细胞。并且细胞识别的抗原的多样性可能受到使用单一V γ的限制。基因片段最后，这些数据提出了一种有趣的可能性，即Thy-1+德克斯可能来自于最先从发育中的胸腺中出现的前体。这表明胸腺细胞发育过程中V基因的使用受到高度调节，对新生细胞的组织定位和功能具有重要影响。与其他发育中的组织一样，程序化和瞬时的基因表达似乎决定了新生细胞的命运。
To study the origin of and the degree of T-cell antigen receptor (TCR) diversity of Thy-1+ dendritic epidermal cells (Thy-1+ dECs) in mice, we have developed a monclonal antibody (mAb 536) to the .gamma..delta. TCR. mAb 536 binds to and stimulates interleukin 2 secretion from Thy-1+ dEC but not cells that express TCR composed of .alpha. and .beta. chains. mAb 536 precipitates CD3-associated .gamma. and .delta. chains from lysates of radioiodinated Thy-1+ dECs. Analysis of a panel of hybridomas that express .gamma..delta. TCR indicated that mAb 536 defines an epitope of the variable region (V.gamma.3) gene product. Flow cytometric anlaysis revealed that expression of V.gamma.3 in the adult mouse is restricted to cells in the epidermis, where essentially all Thy-1+ cells are V.gamma.3+. The majority of CD3+ cells in the 14-day fetal thymus also express V.gamma.3. These results indicate that the T-cell complement in epidermis are cells that express .gamma..delta.TCR and that the diversity of antigens recognized by the cells might be restricted by the use of a single V.gamma. gene segment. Finally, the data raise the intriguing possibility that Thy-1+ dECs may arise from precursors that are among the first to emerge from the developing thymus. This suggests that V gene usage during thymocyte development is highly regulated and has important consequences on the tissue localization and function of the emerging cells. As in other developing tissues, it appears that programmed and transient gene expression determines the fate of the emerging cells.