Oligonol, A New Lychee Fruit-derived Low-molecular Form of Polyphenol, Enhances Lipolysis in Primary Rat Adipocytes through Activation of the ERK1/2 pathway
Oligonol, A New Lychee Fruit-derived Low-molecular Form of Polyphenol, Enhances Lipolysis in Primary Rat Adipocytes through Activation of the ERK1/2 pathway
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DOI：
10.1002/ptr.2846
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发表时间：
2009-11-01
影响因子：
7.2
通讯作者：
Ohno, Hideki
中科院分区：
文献类型：
--
作者：
Ogasawara, Junetsu;Kitadate, Kentaro;Ohno, Hideki
研究了荔枝多酚（LFP）的酚类物质Oligonol对原代脂肪细胞脂解的影响，探讨其调节体内脂肪代谢的可能机制。Oligonol显着增加脂解，这是伴随着细胞外信号相关激酶1/2（ERK 1/2）的激活和围脂蛋白表达下调，而不增加细胞内cAMP的生产。用选择性ERK 1/2抑制剂PD 98059或U 0126预处理可完全阻止Oligonol引起的脂解作用增加，这也阻止了围脂蛋白表达的减少。肿瘤坏死因子-α也下调围脂蛋白的表达。然而，用Oligonol对G α 1蛋白的表达没有显著改变。这些发现表明，Oligonol增强了原代脂肪细胞的脂解，不依赖于cAMP的产生，但其作用依赖于ERK 1/2通路的激活，导致周脂蛋白表达的下调。版权所有（C）2009约翰威利父子有限公司
The effect of Oligonol, a phenolic product from lychee fruit polyphenol (LFP) containing catechin-type monomers and lower oligomers of proanthocyanidin, on lipolysis in primary adipocytes was investigated in order to examine the possible mechanism underlying the regulation of in vivo metabolism in fat. Oligonol significantly increased lipolysis, which was accompanied by both activation of extracellular signaling-related kinase 1/2 (ERK1/2) and down-regulation of perilipin protein expression, without an increase in intracellular cAMP production. The increase in lipolysis with Oligonol was prevented completely by pretreatment with either PD98059 or U0126, selective ERK1/2 inhibitors, which also prevented the reduction in the expression of perilipin protein. Tumor necrosis factor-alpha also down-regulated the expression of perilipin protein. However, there was no significant alteration in the expression of G alpha i protein with Oligonol. These findings indicate that Oligonol enhances lipolysis in primary adipocytes, independent of cAMP production, but its effect is dependent on activation of the ERK1/2 pathway, leading to down-regulation of perilipin protein expression. Copyright (C) 2009 John Wiley & Sons, Ltd.