Cell-surface expression of a mutated Epstein-Barr virus glycoprotein B allows fusion independent of other viral proteins
Cell-surface expression of a mutated Epstein-Barr virus glycoprotein B allows fusion independent of other viral proteins
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DOI：
10.1073/pnas.0404535101
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发表时间：
2004-12-14
影响因子：
11.1
通讯作者：
Longnecker, R
中科院分区：
文献类型：
--
作者：
McShane, MP;Longnecker, R
eb病毒（EBV）感染人类B淋巴细胞和上皮细胞。我们采用无病毒细胞融合实验比较了EBV糖蛋白诱导的中国仓鼠卵巢效应细胞与人B淋巴母细胞或上皮细胞融合的要求。eb病毒编码的gB、gH、gL和gp42糖蛋白可用于B细胞的有效融合，而eb病毒编码的gB、9H和gL糖蛋白可用于中国仓鼠卵巢效应细胞与上皮细胞系（AGS和SCC68）或人胚胎肾细胞系293-P的有效融合。与人胚胎肾293-P细胞的融合大于与B细胞的融合，表明细胞接触的重要作用。一种针对gH和gL复合物的抗体抑制上皮细胞融合。由于羧基末端尾部的截断或点突变，单独的gB表面表达增加，使得gB介导的与上皮细胞融合，尽管其水平低于gB、gH和gL的共表达。总的来说，gB似乎是EBV糖蛋白介导的细胞融合的关键成分。
Epstein-Barr virus (EBV) infects human B lymphocytes and epithelial cells. We have compared the requirements for EBV glycoprotein-induced cell fusion between Chinese hamster ovary effecter cells and human B lymphoblasts or epithelial cells by using a virus-free cell fusion assay. EBV-encoded gB, gH, gL, and gp42 glycoproteins were required for efficient B cell fusion, whereas EBV gB, 9H, and gL glycoproteins were required for Chinese hamster ovary effecter cell fusion with epithelial cell lines (AGS and SCC68) or the human embryonic kidney cell line 293-P. Fusion with human embryonic kidney 293-P cells was greater than fusion observed with B cells, indicative of an important role for cell contact. An antibody directed against the gH and gL complex inhibited epithelial cell fusion. Increased surface expression of gB alone as a result of truncations or point mutants in the carboxyl-terminal tail allowed gB-mediated fusion with epithelial cells, albeit at a lower level than with coexpression of gB, gH, and gL. Overall, gB appears to be the critical component for EBV glycoprotein-mediated cell fusion.