Essential amino acid and carbohydrate supplementation ameliorates muscle protein loss in humans during 28 days bedrest
Essential amino acid and carbohydrate supplementation ameliorates muscle protein loss in humans during 28 days bedrest
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DOI：
10.1210/jc.2003-032159
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发表时间：
2004-09-01
影响因子：
5.8
通讯作者：
Ferrando, AA
中科院分区：
文献类型：
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作者：
Paddon-Jones, D;Sheffield-Moore, M;Ferrando, AA
我们确定了补充必需氨基酸和碳水化合物是否可以抵消长期不活动的分解代谢反应。主要结果指标包括混合肌合成率（FSR）、苯丙氨酸净平衡、瘦腿质量和腿伸展强度。在第1天和第28天，在预充的L-[环-H-2（5）]苯丙氨酸持续输注期间获得股外侧肌活检和股动静脉血样。计算16 h内的净平衡和FSR，在此期间，对照组（CON）每5 h（0830，1330和1830 h）接受营养混合餐。实验组（EXP）还消耗16.5g必需氨基酸和30 g碳水化合物（1100、1600和2100 h）。卧床期间维持饮食方案。EXP组在第1天（EXP，0.099 +/- 0.008%/ h; CON：0.075 +/- 0.005%/h）和第28天（EXP，0.093 +/- 0.006%/ h; CON，0.055 +/- 0.007%/ h）的FSR较高。EXP组在整个卧床期间保持了瘦腿质量（+0.2 +/-0.3 kg），但CON组下降（-0.4 +/-0.1 kg）。CON组的力量损失更明显（EXP，-8.8 +/- 1.4 kg; CON，-17.8 +/- 4.4 kg）。补充必需氨基酸和碳水化合物可能是一种可行的干预措施，对因不动或长期卧床而有肌肉减少症风险的个体。
We determined whether essential amino acid and carbohydrate supplementation could offset the catabolic response to prolonged inactivity. Major outcome measures included mixed muscle fractional synthetic rate (FSR), phenylalanine net balance, lean leg mass, and leg extension strength. On d 1 and 28, vastus lateralis muscle biopsies and femoral arteriovenous blood samples were obtained during a primed constant infusion of L-[ring-H-2(5)] phenylalanine. Net balance and FSR were calculated over 16 h, during which the control group (CON) received a nutritionally mixed meal every 5 h ( 0830, 1330, and 1830 h). The experimental group (EXP) also consumed 16.5 g essential amino acids and 30 g carbohydrate 1100, 1600, and 2100 h). The dietary regimen was maintained during bedrest. FSR was higher in the EXP group on d 1 (EXP, 0.099 +/- 0.008%/ h; CON: 0.075 +/- 0.005%/h) and d 28 (EXP, 0.093 +/- 0.006%/ h; CON, 0.055 +/- 0.007%/ h). Lean leg mass was maintained throughout bedrest in the EXP group (+0.2 +/- 0.3 kg), but fell in the CON group (-0.4 +/- 0.1 kg). Strength loss was more pronounced in the CON group (EXP, -8.8 +/- 1.4 kg; CON, - 17.8 +/- 4.4 kg). Essential amino acid and carbohydrate supplementation may represent a viable intervention for individuals at risk of sarcopenia due to immobility or prolonged bedrest.