The more important role of archaea than bacteria in nitrification of wastewater treatment plants in cold season despite their numerical relationships.
The more important role of archaea than bacteria in nitrification of wastewater treatment plants in cold season despite their numerical relationships.
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DOI：
10.1016/j.watres.2018.08.066
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发表时间：
2018-11
期刊：
影响因子：
12.8
通讯作者：
Kai-ling Pan;Jingfeng Gao;Xiaoyan Fan;Dingchang Li;Huihui Dai
中科院分区：
文献类型：
--
作者：
Kai-ling Pan;Jingfeng Gao;Xiaoyan Fan;Dingchang Li;Huihui Dai
Nitrification failure of wastewater treatment plants (WWTPs) in cold season calls into investigations of the functional ammonia-oxidizing microorganisms (AOMs). In this study, we report the abundance of ammonia-oxidizing archaea (AOA), bacteria (AOB) and complete ammonia-oxidizing (comammox)Nitrospirain 23 municipal WWTPs in cold season, and explore the correlations between AOMs abundance and their relative contribution to nitrification. The copy numbers of AOA and AOBamoAgene ranged from 2.42 × 107to 2.47 × 109and 5.54 × 106to 3.31 × 109copies/g sludge, respectively. The abundance ofamoAgene ofCandidatusNitrospira inopinata, an important strain of comammoxNitrospira, was stable with averaged abundance of 8.47 × 106copies/g sludge. DNA-based stable isotope probing (DNA-SIP) assays were conducted with three typical WWTPs in which the abundance of AOA was lower than, similar to and higher than that of AOB, respectively. The results showed that considerable13C-assimilation by AOA was detected during active nitrification in all WWTPs, whereas just a much lesser extent of13C-incorporation by AOB and comammoxNitrospirawas found in one WWTP. High-throughput sequencing with13C-labeled DNA also showed the higher reads abundance of AOA than AOB and comammoxNitrospira.Nitrososphaera viennensiswas the dominant active AOA, whileNitrosomonas oligotrophaandNitrosomonas europaeawere identified as active AOB. The results obtained suggest that AOA, rather than AOB and comammoxNitrospira, dominate ammonia oxidation in WWTPs in cold season despite the numerical relationships of AOMs.
