Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes.
Mycobacteria, but not mercury, induces metallothionein (MT) protein in striped bass, Morone saxitilis, phagocytes, while both stimuli induce MT in channel catfish, Ictalurus punctatus, phagocytes.
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分枝杆菌（而非汞）会在条纹鲈、莫罗尼拟鲈、吞噬细胞中诱导金属硫蛋白 (MT) 蛋白，而两种刺激物都会在斑点叉尾鮰、斑点叉尾鮰、吞噬细胞中诱导 MT。
DOI：
10.1016/j.marenvres.2004.03.021
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Rice,CD
中科院分区：
文献类型：
--
作者：
Regala,RP;Rice,CD
分子免疫学的最新进展表明，诱导炎性蛋白的表达在脊椎动物对感染性疾病因子和各种异种生物的反应中增加。例如，iNOS、COX-2和CYP1A可被炎症和AhR配体诱导。此外，这些蛋白的表达对刺激的反应在群体中的个体之间是不同的。鱼类对感染因子和外源药物诱导促炎蛋白的差异知之甚少。通过随机筛选条纹鲈鱼、摩罗鱼、腹膜巨噬细胞cDNA文库，克隆出金属硫蛋白（MT）全长基因并进行测序。MT是一种低分子量（6-8 kDa）、富含半胱氨酸的金属结合蛋白。致病菌生长需要金属，宿主防御系统也需要金属，因为金属是吞噬细胞生成活性氧中间体（ROIs）的催化剂。表达并纯化了重组条纹鲈MT (rMT)，制备了特异性单抗（MT-16）。在体外暴露于自然存在的细胞内病原体福氏分枝杆菌（mycobacterium fortuitum）或0.1和1 μM汞（Hg） （HgCl2）后，新分离的条纹鲈和通道鲶鱼（Ictalurus punctatus）吞噬细胞中观察MT蛋白的表达。在通道鲶鱼和条纹鲈鱼的吞噬细胞中，MT的表达均因暴露于M. fortunitum细胞而增加了24 h。另一方面，汞在通道鲶鱼细胞中诱导MT，而在条纹鲈鱼细胞中不诱导。这些结果表明，鲶鱼和条纹鲈鱼的吞噬细胞中金属稳态存在差异。此外，这些数据表明，当使用MT表达作为金属暴露的生物标志物时，应注意区分炎症诱导的MT和金属诱导的MT。
Recent advances in molecular immunology indicate that the expression of inducible pro-inflammatory proteins is increased in vertebrates in response to both infectious disease agents and various xenobiotics. For example, iNOS, COX-2, and CYP1A are induced by both inflammation and AhR ligands. Moreover, the expression of these proteins in response to stimuli varies among individuals within populations. Little is known of the differences among fish in the inducibility of proinflammatory proteins in response to both infectious agents and xenobiotics. Through random screening of a striped bass, Morone saxitilis, peritoneal macrophage cDNA library, a full length metallothionein (MT) gene was cloned and sequenced. MT is a low-molecular weight (6–8 kDa), cysteine-rich metal binding protein. Metals are required by pathogenic bacteria for growth, and by the host defense system by serving as a catalyst for the generation of reactive oxygen intermediates (ROIs) by phagocytes. A recombinant striped bass MT (rMT) was expressed and purified, then used to generate a specific mAb (MT-16). MT protein expression was followed in freshly isolated striped bass and channel catfish, Ictalurus punctatus, phagocytes after in vitro exposure to the naturally occurring intracellular pathogen Mycobacteria fortuitum or to 0.1 and 1 μM mercury (Hg), as HgCl2. MT expression was increased by 24 h in both channel catfish and striped bass phagocytes as a result of exposure to M. fortuitum cells. On the other hand, MT was induced by Hg in channel catfish cells, but not those of striped bass. These results indicate that metal homeostasis in phagocytes is different between catfish and striped bass. In addition, these data suggest that care should be taken to distinguish between inflammation-induced vs. metal-induced MT when using MT expression as a biomarker of metal exposure.