Expression analysis of the Toll-like receptor and TIR domain adaptor families of zebrafish
Expression analysis of the Toll-like receptor and TIR domain adaptor families of zebrafish
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DOI：
10.1016/j.molimm.2003.10.003
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发表时间：
2004-01-01
影响因子：
3.6
通讯作者：
Spaink, HP
中科院分区：
文献类型：
--
作者：
Meijer, AH;Krens, SFG;Spaink, HP
分析斑马鱼基因组序列数据库中存在toll样受体（TLR）和白细胞介素受体（IL-R）成员的编码基因，以及包含TIR结构域的相关衔接蛋白。结果预测显示，存在一个或多个人类TLR1、TLR2、TLR3、TLR4、TLR5、TLR7、TLR8、TLR9、IL- 1R和IL18R基因的对应基因，以及一个适配器基因MyD88、MAL、TRIF和SARM的拷贝。与河豚相比，斑马鱼有两个与人类TLR4高度相似的基因。此外，还可以区分出一个与黑腹果蝇Toll-9基因密切相关的鱼类特异性TLR群。克隆的TLR4， TLR2和MyD88的cdna序列显示出与人类对应的相同的内含子-外显子组织。逆转录- pcr （RT-PCR）表达分析显示，17个预测的斑马鱼TLR基因和所有编码接头蛋白的基因在成虫期均有表达。TLR基因的一个子集在感染致病菌海洋分枝杆菌的鱼类中表达水平较高。诱导基因包括人类TLR1和TLR2基因的同源物，其功能与分枝杆菌感染相关，强调了斑马鱼作为脊椎动物先天免疫系统分析模型的适用性。(C) 2003 Elsevier Ltd.版权所有。
The zebrafish genomic sequence database was analysed for the presence of genes encoding members of the Toll-like receptors (TLR) and interleukin receptors (IL-R) and associated adaptor proteins containing a TIR domain. The resulting predictions show the presence of one or more counterparts for the human TLR1, TLR2, TLR3, TLR4, TLR5, TLR7, TLR8, TLR9, IL- 1R and IL18R genes and one copy of the adaptor genes MyD88, MAL, TRIF and SARM. In contrast to data for the pufferfish Fugu rubripes, zebrafish has two genes that are highly similar to human TLR4. In addition, one fish-specific TLR group can be distinguished that is closely related to the Drosopohila melanogaster Toll-9 gene. The sequence of cloned cDNAs for TLR4, TLR2 and MyD88 show the same intron-exon organisation as in the human counterparts. Expression analysis using reverse transcriptase-PCR (RT-PCR) shows that 17 of the predicted zebrafish TLR genes and all the genes encoding adaptor proteins are expressed in the adult stage. A subset of the TLR genes are expressed at higher levels in fish infected with the pathogen Mycobacterium marinium. The induced genes include the homologues of the human TLR1 and TLR2 genes, whose functions are associated with mycobacterial infections, underscoring the suitability of zebrafish as a model for analysis of the vertebrate innate immune system. (C) 2003 Elsevier Ltd. All rights reserved.