Embryo implantation after biopsy of one or two cells from cleavage‐stage embryos with a view to preimplantation genetic diagnosis
Embryo implantation after biopsy of one or two cells from cleavage‐stage embryos with a view to preimplantation genetic diagnosis
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对卵裂期胚胎的一个或两个细胞进行活检后进行胚胎植入，以进行植入前遗传学诊断
DOI：
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发表时间：
2000
影响因子：
3
通讯作者：
A. V. Van Steirteghem
中科院分区：
文献类型：
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作者：
H. Van de Velde;A. De Vos;K. Sermon;C. Staessen;M. De Rycke;E. van Assche;W. Lissens;M. Vandervorst;H. Van Ranst;I. Liebaers;A. V. Van Steirteghem
胚胎植入前遗传学诊断 (PGD) 可以作为产前诊断 (PND) 的替代方案，为有可能生育患有遗传病的孩子的夫妇提供。受影响的胚胎在植入前通过荧光原位杂交 (FISH) 检测性别鉴定（X 连锁疾病）和染色体疾病（数量和结构），或通过聚合酶链反应 (PCR) 检测单基因疾病（包括一些 X 连锁疾病）。通过分析胚胎的两个卵裂球，可以提高诊断的准确性和可靠性。然而，去除两个卵裂球可能会影响胚胎的着床能力。我们评估了在进行移植的 188 个 PGD 周期中移除一个、两个或三个细胞后胚胎的植入情况。患者被分为五组：第一组仅接受已去除一个细胞的胚胎，第二组仅接受已去除两个细胞的胚胎，第三组接受已去除一个和两个细胞的胚胎混合物，第四组已去除两个和三个细胞，第五组已去除三个细胞。每次移植的总体持续妊娠率为 26.1%，每次移植的总体着床率为 15.2%，总体出生率为 14.2%。尽管由于第二组（去除两个细胞）包含发育更快、因此“质量更好”的胚胎，因此无法比较各组之间的妊娠率，但该组中每个移植胚胎 29.1% 的持续妊娠率和 18.6% 的着床率是可以接受的，因此我们建议分析来自 ≥7 细胞阶段胚胎的两个细胞，以使诊断更加准确和可靠。版权所有 © 2000 约翰·威利父子有限公司
Preimplantation genetic diagnosis (PGD) can be offered as an alternative to prenatal diagnosis (PND) to couples at risk of having a child with a genetic disease. The affected embryos are detected before implantation by fluorescent in situ hybridisation (FISH) for sexing (X‐linked diseases) and chromosomal disorders (numerical and structural) or by polymerase chain reaction (PCR) for monogenic disorders (including some X‐linked diseases). The accuracy and reliability of the diagnosis is increased by analysing two blastomeres of the embryo. However, the removal of two blastomeres might have an effect on the implantation capacity of the embryo. We have evaluated the implantation of embryos after the removal of one, two or three cells in 188 PGD cycles where a transfer was done. The patients were divided into five groups: a first group which received only embryos from which one cell had been removed, a second group which received only embryos from which two cells had been removed, a third group which received a mixture of embryos from which one and two cells had been taken, a fourth group where two and three cells had been removed, and a fifth group where three cells had been removed. The overall ongoing pregnancy rate per transfer was 26.1%, the overall implantation rate per transfer was 15.2% and the overall birth rate was 14.2%. Although pregnancy rates between the groups cannot be compared because the second group (two cells removed) contains more rapidly developing and therefore ‘better quality’ embryos, an ongoing pregnancy rate of 29.1% and an implantation rate of 18.6% per transferred embryo in this group is acceptable, and we therefore advise analysing two cells from a ≥7‐cell stage embryo in order to render the diagnosis more accurate and reliable. Copyright © 2000 John Wiley & Sons, Ltd.
影响因子：
6.1
作者：
Antczak, M;Van Blerkom, J
通讯作者：
Van Blerkom, J