Characterization of hydrocarbon-degrading bacteria isolated from oil-contaminated sediments in the Sultanate of Oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments
Characterization of hydrocarbon-degrading bacteria isolated from oil-contaminated sediments in the Sultanate of Oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments
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DOI：
10.1016/j.ibiod.2014.01.006
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发表时间：
2014-04-01
影响因子：
4.8
通讯作者：
Al-Hinai, Manal
中科院分区：
文献类型：
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作者：
Abed, Raeid M. M.;Al-Sabahi, Jamal;Al-Hinai, Manal
从阿曼沿海地区采集了两个受石油污染的沉积物(Pd和KH)，以分离碳氢化合物降解菌并测试不同的生物修复方法。共分离到40株细菌，其中18株隶属于海洋杆菌属，其余为假单胞菌属、盐生单胞菌属、哈氏杆菌属和炭疽杆菌属。所有菌株在2-7%的盐度和20-60℃之间生长良好，在长链烷烃上的生长好于在短链烷烃上的生长。对两种沉积物中的生物刺激和生物强化作用进行了比较，并通过测定二氧化碳的释放和气相色谱(GC)对原油进行了生物降解。在未经处理的Pd和KH沉积物中，88天后释放出的CO2分别达到0.45+/-0.02和2.23+/-0.07 mg CO2，g(-1)。虽然无机营养物质的添加使两种沉积物中的CO2释放增加了1.2-3.7倍，但添加细菌联合体只对Pd沉积物有效。当同时添加营养物质和细菌时，测得的最大CO2释放量相当于88天后PD和KH沉积物中总石油矿化量的2.6+/-0.12%和1.49+/-0.04%。GC分析证实了CO2的数据，并表明大多数降解化合物属于烷烃。我们的结论是，阿曼污染的沉积物中含有耐盐性和耐热性细菌，生物刺激比生物增强更能有效地清除这些细菌。(C)2014爱思唯尔有限公司。保留所有权利。
Two oil-polluted sediments (PD and KH) were sampled from a coastal region in Oman for the isolation of hydrocarbon-degrading bacteria and for testing different bioremediation approaches. Fourty strains were isolated, eighteen were affiliated to Marinobacter whereas the rest belonged to Pseudomonas, Halomonas, Hahella and Alcanivorax. All strains grew well at 2-7% salinity and between 20 and 60 degrees C. The strains exhibited a better growth on long chain than on short chain alkanes. Biostimulation and bioaugmentation were compared in both sediments and oil biodegradation was followed by measuring CO2 evolution and by gas chromatography (GC). The evolved CO2 reached 0.45 +/- 0.02 and 2.23 +/- 0.07 mg CO2, g(-1), sediment after 88 days in the untreated PD and KH sediments, respectively. While the addition of inorganic nutrients resulted in 1.2-3.7 fold increase in CO2 evolution in both sediments, the addition of the bacterial consortium was only effective in the PD sediment. The maximum CO2 evolution was measured when both nutrients and bacteria were added and this corresponded to a total oil mineralization of 2.6 +/- 0.12 and 1.49 +/- 0.04% of the initial oil after 88 days in the PD and KH sediments, respectively. GC analysis confirmed the CO2 data and showed that most of the degraded compounds belonged to alkanes. We conclude that the Omani polluted sediments contain halotolerant and thermotolerant bacteria and biostimulation is more efficient than bioaugmentation for their cleanup. (C) 2014 Elsevier Ltd. All rights reserved.