Spectrally resolved confocal microscopy using lanthanide centred near-IR emission
Spectrally resolved confocal microscopy using lanthanide centred near-IR emission
复制标题
DOI：
10.1039/c4cc09618e
复制
发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Sorensen, Thomas Just
中科院分区：
文献类型：
--
作者：
Liao, Zhiyu;Tropiano, Manuel;Sorensen, Thomas Just
镧系离子的窄近红外（NIR）发射引起了极大的兴趣，特别是在开发生物成像工具方面，其中镧系元素激发态的长寿命可以用来解决由自身荧光和样品透明度引起的问题。尽管基于镧系元素的探测器有望用于近红外成像，但文献中很少有关于其使用的报道。在这里，我们证明了图像可以通过使用探测器、光学元件和显微镜来监测镧系化合物的近红外发射，这些探测器、光学元件和显微镜主要是为光谱的可见部分设计的。
The narrow, near infrared (NIR) emission from lanthanide ions has attracted great interest, particularly with regard to developing tools for bioimaging, where the long lifetimes of lanthanide excited states can be exploited to address problems arising from autofluorescence and sample transparency. Despite the promise of lanthanide-based probes for near-IR imaging, few reports on their use are present in the literature. Here, we demonstrate that images can be recorded by monitoring NIR emission from lanthanide complexes using detectors, optical elements and a microscope that were primarily designed for the visible part of the spectrum.