Detection and Quantification of Nitric Oxide Using Electron Magnetic Resonance Spectroscopy
Detection and Quantification of Nitric Oxide Using Electron Magnetic Resonance Spectroscopy
复制标题
使用电子磁共振波谱法检测和定量一氧化氮
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
Roger P. Smith
中科院分区：
文献类型：
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作者：
D. Wilcox;Roger P. Smith
摘要 综述了使用电子磁共振 (EMR) 光谱（也称为电子顺磁共振 (EPR) 或电子自旋共振 (ESR) 光谱）来检测和定量模型系统、细胞和活体有机体中的顺磁性分子一氧化氮 (NO) 的情况。介绍了 EMR 波谱的物理基础以及该方法提供的信息。由于溶液中的 EMR 无法检测到游离 NO，因此总结了在顺磁 EMR 可检测复合物中捕获和稳定 NO 的化合物和方法（通常 NO 与 Fe(II) 结合），以及使用 EMR 光谱定量 NO 的其他间接方法。讨论了样品操作、数据收集和数据分析，特别是 EMR 可检测的 NO 复合物。作者自己的工作中提供了示例，说明了 EMR 波谱在 NO 检测和定量方面的不同应用。最后，将 EMR 与其他测定生物和模型系统中 NO 的方法进行了比较。
Abstract The use of electron magnetic resonance (EMR) spectroscopy, also known as electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy, to detect and quantify the paramagnetic molecule nitric oxide, NO, in model systems, cells, and living organisms is reviewed. The physical basis for EMR spectroscopy and the information available from this method are presented. Since free NO is not detectable by EMR in solution, compounds and methods for trapping and stabilizing NO in paramagnetic EMR-detectable complexes, often with NO bound to Fe(II), are summarized, as are other indirect methods for quantifying NO with EMR spectroscopy. Sample manipulation, data collection, and data analysis are discussed, with particular reference to EMR-detectable NO complexes. Examples are presented from the author′s own work that Illustrate different applications of EMR spectroscopy for detection and quantification of NO. Finally, EMR is compared to other methods for the determination of NO in biological and model systems.