Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells
Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells
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DOI：
10.1097/cad.0b013e328223f14d
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发表时间：
2007-10-01
影响因子：
2.3
通讯作者：
Xian, Li-Jian
中科院分区：
文献类型：
--
作者：
Wang, Lin;Sun, Jian;Xian, Li-Jian
本研究以多药耐药的人表皮样C-All 20细胞和敏感的亲本KB细胞作为实验模型。BIVI-细胞周期蛋白1，一种传统的抗支原体药物，进行了测试，以探讨在这些细胞系的多药耐药的逆转作用及其机制。MTT结果显示BM-cyclin 1能有效逆转C-A120细胞的多药耐药，使C-A120细胞对阿霉素、足叶乙甙和顺铂的敏感性分别提高6.0、8.2和1.7倍。免疫印迹分析和逆转录-聚合酶链反应用于研究BIVI-细胞周期蛋白1诱导的拓扑异构酶Ila的变化。结果表明，拓扑异构酶Ⅱ α在C-A120细胞中的表达明显增加。拓扑异构酶11的催化活性增加了30%，与未经处理的细胞相比，通过decatenation kinetopolast DNA测量。免疫印迹分析也表明特异性转录因子水平：蛋白1（Sp1）和核因子-YA与BIVI-细胞周期蛋白1治疗后增加，而多药耐药蛋白2的mRNA和蛋白表达显着下调。这些结果表明，BM-cyclin 1可以通过增加拓扑异构酶II α的表达和抑制多药耐药蛋白2的表达，有效地逆转C-All 20细胞的多药耐药，强烈提示BM-cyclin 1是一种潜在的多药耐药逆转剂。(c)2007年利平科特威廉姆斯&威尔金斯。
In this study, multidrug-resistant human epidermoid C-All 20 cells and the sensitive parental KB cells were used as experimental models. BIVI-cyclin 1, a traditional antimycoplasma drug, was tested to explore the reversal effect of multidrug resistance and its mechanisms in these cell lines. The MTT analysis showed that BM-cyclin 1 could reverse multidrug resistance effectively in C-A120 cells; the sensitivity of C-A120 cells to adriamycin, etoposide and cisplatin was enhanced by 6.0, 8.2 and 1.7 times, respectively. Immunoblotting analysis and reverse transcription-polymerase chain reaction were used to study the BIVI-cyclin 1-induced changes in topoisomerase I la. The results showed that the expression of topoisomerase II alpha in treated C-A120 cells increased significantly. Topoisomerase 11 catalytic activity increased by 30% compared with the untreated cells, as measured by decatenation of kinetopolast DNA. Immunoblotting analysis also indicated the transcription factor levels of specificity: those of protein 1 (Sp1) and nuclear factor-YA increased after treatment with BIVI-cyclin 1, whereas the mRNA and protein expression of multidrug resistance protein 2 was significantly downregulated. These results demonstrated that BM-cyclin 1 could effectively reverse the multidrug resistance of C-All 20 cells by increasing the expression of topoisomerase II alpha and by suppressing the expression of multidrug resistance protein 2, strongly suggesting that BM-cyclin 1 is a potential multidrug resistance reversal agent. (c) 2007 Lippincott Williams & Wilkins.