Abietane diterpenoids from Salvia officinalis leaves as aryl hydrocarbon receptor ligands
Abietane diterpenoids from Salvia officinalis leaves as aryl hydrocarbon receptor ligands
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DOI：
10.1016/j.phytol.2020.11.006
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发表时间：
2021-02-01
影响因子：
1.7
通讯作者：
Nagao, Masaya
中科院分区：
文献类型：
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作者：
Nishino, Katsutoshi;Someya, Kenta;Nagao, Masaya
从地中海地区民间药用鼠尾草的叶中分离得到1个未知的和6个已知的松烷二萜类化合物作为芳烃受体(AhR)的配体。通过1D/2D核磁共振波谱和高分辨电喷雾质谱确证了该化合物的结构，命名为(+)-(10R)-11，14-dioxoabieta-8，12-diene-12，20-diol。用稳定转染HepG2细胞的AhR依赖的荧光素酶报告系统(HepG2-XRE)检测分离的二萜类化合物的AhR配体活性。从丹参中提取的每个AhR配体在30 mU M处都显示出AhR配体活性。
One undescribed and six known abietane diterpenoids were isolated as ligands for aryl hydrocarbon receptor (AhR) from leaves of Salvia officinalis, a folk medicine in the Mediterranean Sea area. The structure of the undescribed diterpenoid named (+)-(10R)-11,14-dioxoabieta-8,12-diene-12,20-diol was assigned by 1D/2D NMR spectroscopic and HR-ESI-MS data. The AhR ligand activity of the isolated diterpenoids was evaluated by AhR-dependent luciferase reporter system stably transfected in HepG2 cells (HepG2-XRE). Each AhR ligand derived from Salvia officinalis exhibited AhR ligand activity at 30 mu M.