Insulin-like growth factor I in the dog: a study in different dog breeds and in dogs with growth hormone elevation.
Insulin-like growth factor I in the dog: a study in different dog breeds and in dogs with growth hormone elevation.
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狗体内的胰岛素样生长因子 I：对不同品种的狗和生长激素升高的狗的研究。
DOI：
10.1530/acta.0.1050294
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
E. Froesch
中科院分区：
文献类型：
--
作者：
J. Eigenmann;D. Patterson;J. Zapf;E. Froesch
采用放射免疫法（RIA）测定人胰岛素样生长因子I (IGF I)，测定犬的IGF I。犬的IGF I通过凝胶层析法从血浆标本中提取。用1 M乙酸洗脱柱，将层积为55 - 85%的部分混合，冷冻干燥，用测定缓冲液重组。连续稀释犬IGF我从正常和肢端肥大症的狗当添加到RIA系统做了一个类似的位移模式的人类[125 I] IGF我通过添加未标记的人类的IGF I剂量反应曲线获得的犬IGF我平行的人类获得的IGF I Logit-log变换和最小二乘拟合导致之间的直线拟合标准曲线0.039和5 ng IGF每管我添加了。试验内变异系数（CV）为16.7%，试验间变异系数为21.8%。正常狗的血浆IGF - I浓度似乎与体型有关。可卡犬的浓度为36 +/- 27 ng/ml，比格犬为87 +/- 33 ng/ml，基山犬为117 +/- 34 ng/ml，德国牧羊犬为280 +/- 23 ng/ml（平均+/- SEM）。生长激素（GH）升高犬组的平均IGF - I水平为700 +/- 90 ng/ml。虽然这组狗包括小型和大型狗，但平均IGF - I水平与研究中最大的品种德国牧羊犬的水平显著不同（P < 0.01）。（摘要删节250字）
A radioimmunoassay (RIA) devised for the measurement of human insulin-like growth factor I (IGF I) was employed for the measurement of canine IGF I. Canine IGF I was extracted from plasma specimens by gel chromatography. Columns were eluted with 1 M acetic acid and the fractions representing the 55 to 85% bed volume were pooled, lyophilized and reconstituted with assay buffer. Serial dilutions of canine IGF I from both normal and acromegalic dogs when added to the RIA system gave a similar displacement pattern of human [125I]IGF I as the one obtained by the addition of unlabelled human IGF I. The dose-response curve obtained by canine IGF I paralleled the one obtained by human IGF I. Logit-log transformation and least squares fitting resulted in straight line fitting of the standard curve between 0.039 and 5 ng IGF I added per tube. The within-assay coefficient of variation (CV) was 16.7% and the between-assay CV was 21.8%. Plasma IGF I concentrations in normal dogs appeared to be a function of body size. The concentrations were 36 +/- 27 ng/ml in Cocker Spaniels, 87 +/- 33 ng/ml in Beagles, 117 +/- 34 ng/ml in Keeshonds, and 280 +/- 23 ng/ml in German Shepherds (mean +/- SEM). The mean IGF I level in a group of dogs with growth hormone (GH) elevation was 700 +/- 90 ng/ml. Though this group of dogs comprised both small and large dogs, the mean IGF I level significantly differed from the one found in German Shepherds, the largest breed studied (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)