Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.
Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts.
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成年犬心脏心球来源细胞的心脏再生潜力
DOI：
10.1111/jcmm.12585
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发表时间：
2015-08
影响因子：
5.3
通讯作者：
Cheng K
中科院分区：
文献类型：
--
作者：
Hensley MT;de Andrade J;Keene B;Meurs K;Tang J;Wang Z;Caranasos TG;Piedrahita J;Li TS;Cheng K
心肌球衍生细胞（CDC）的再生潜力，缺血性心脏病已被证明在小鼠，大鼠，猪和最近完成的临床试验。来自狗心脏的CDC的再生潜力尚未得到测试。在这里，我们表明，犬CDC可以从成年狗的心脏。这些细胞与先前研究的来自啮齿动物和人类的CDC表现出相似的表型。犬CDCs在体外可分化为心肌细胞、平滑肌细胞和内皮细胞。此外，来自犬CDC的条件培养基促进血管生成但抑制心肌细胞死亡。在阿霉素诱导的扩张型心肌病（DCM）小鼠模型中，静脉输注犬CDC可改善心脏功能并减少心脏纤维化。组织学显示，注射的犬CDCs在小鼠心脏中移植并增加毛细血管密度。我们的研究证明了犬CDC在DCM小鼠模型中的再生潜力。
The regenerative potential of cardiosphere-derived cells (CDCs) for ischaemic heart disease has been demonstrated in mice, rats, pigs and a recently completed clinical trial. The regenerative potential of CDCs from dog hearts has yet to be tested. Here, we show that canine CDCs can be produced from adult dog hearts. These cells display similar phenotypes in comparison to previously studied CDCs derived from rodents and human beings. Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro. In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death. In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis. Histology revealed that injected canine CDCs engraft in the mouse heart and increase capillary density. Out study demonstrates the regenerative potential of canine CDCs in a mouse model of DCM.