Sensory axons induce epithelial lipid microdomain remodeling and determine the distribution of junctions in the epidermis.
Sensory axons induce epithelial lipid microdomain remodeling and determine the distribution of junctions in the epidermis.
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DOI：
10.1091/mbc.e22-09-0396
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发表时间：
2023-01-01
影响因子：
3.3
通讯作者：
Sagasti, Alvaro
中科院分区：
文献类型：
--
作者：
Rosa, Jeffrey B.;Nassman, Khaled Y.;Sagasti, Alvaro
上皮细胞的特性是由膜脂、细胞骨架和粘附连接的极化分布决定的。上皮细胞通常有丰富的神经支配，但很少有研究表明神经突如何影响上皮组织。我们之前发现斑马鱼表皮的基底角化细胞包围轴突，形成由自典型连接封闭的鞘通道。在这里，我们描述了轴突如何重塑细胞膜、细胞骨架和基底角化细胞的连接。在基底角化细胞的顶端表面，轴突组织的脂质微域在PI(4,5)P2和液体有序（Lo）膜的报告中定量富集。脂质微域支持富含钙粘蛋白的f -肌动蛋白突起的形成，这些突起包裹在轴突周围，可能启动了鞘层。在没有轴突的情况下，在基底细胞上形成了富含钙粘蛋白的微结构域，但没有组织成连续的结构域。相反，这些孤立的结构域与外周细胞（一种独特的上皮细胞类型）形成异型连接。因此，轴突末梢显著地重塑极化上皮成分并调节表皮粘附。
Epithelial cell properties are determined by the polarized distribution of membrane lipids, the cytoskeleton, and adhesive junctions. Epithelia are often profusely innervated, but little work has addressed how neurites affect epithelial organization. We previously found that basal keratinocytes in the zebrafish epidermis enclose axons in ensheathment channels sealed by autotypic junctions. Here we characterized how axons remodel cell membranes, the cytoskeleton, and junctions in basal keratinocytes. At the apical surface of basal keratinocytes, axons organized lipid microdomains quantitatively enriched in reporters for PI(4,5)P2 and liquid-ordered (Lo) membranes. Lipid microdomains supported the formation of cadherin-enriched, F-actin protrusions, which wrapped around axons, likely initiating ensheathment. In the absence of axons, cadherin-enriched microdomains formed on basal cells but did not organize into contiguous domains. Instead, these isolated domains formed heterotypic junctions with periderm cells, a distinct epithelial cell type. Thus, axon endings dramatically remodel polarized epithelial components and regulate epidermal adhesion.
影响因子：
3.7
作者：
Kurrle N;Völlner F;Eming R;Hertl M;Banning A;Tikkanen R
通讯作者：
Tikkanen R