α6 integrin is the main receptor of human papillomavirus type 16 VLP
α6 integrin is the main receptor of human papillomavirus type 16 VLP
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DOI：
10.1006/bbrc.2001.4838
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发表时间：
2001-05-11
影响因子：
3.1
通讯作者：
Cheong, SW
中科院分区：
文献类型：
--
作者：
Yoon, CS;Kim, KD;Cheong, SW
本研究使用重组人乳头瘤病毒样颗粒（HPV-16 L1-VLP）来确定 HPV-16 型的特异性受体。通过流式细胞术测定 α (6)、β (1) 和 β (4) 整合素的表达水平，并与 10 个细胞系中 HPV-VLP 结合的量进行比较。我们的结果表明，VLP 结合量和 α (6) 整合素的表达水平是相关的，这通过使用抗体的抑制实验和免疫细胞化学得到了证实。宫颈癌细胞系中α(6)整合素的表达水平和HPV-VLP结合量均较高，因为HPV-16型是宫颈癌的主要原因。 HPV-VLP的结合程度与细胞系中的α(6)整合素表达水平相匹配，但与β(1)和β(4)水平不相关，这表明a(6)整合素是HPV 16型的主要受体。(C)2001学术出版社。
The present study was performed to determine the specific receptor of type HPV-16 using recombinant human papillomavirus-like particle (HPV-16 L1-VLP). The expression levels of alpha (6), beta (1), and beta (4) integrins were determined and compared with the amount of HPV-VLP binding in ten cell lines by flow cytometry. Our results show that the amount of VLP binding and the expression level of alpha (6) integrin are correlated, which was confirmed by an inhibition experiment using antibodies and by immunocytochemistry. Both the expression level of alpha (6) integrin and the amount of HPV-VLP binding were high in cervical cancer cell lines, as the type HPV-16 is the main cause of cervical cancer. The degree of binding of HPV-VLP matched the alpha (6) integrin expression level in cell lines but was not correlated with beta (1) and beta (4) levels, which suggests that a(6) integrin is the main receptor of HPV type 16. (C) 2001 Academic Press.