Intracellularly labeled pyramidal neurons in the cortical areas projecting to the spinal cord - I. Electrophysiological properties of pyramidal neurons
Intracellularly labeled pyramidal neurons in the cortical areas projecting to the spinal cord - I. Electrophysiological properties of pyramidal neurons
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DOI：
10.1016/j.neures.2004.08.006
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发表时间：
2004-12-01
影响因子：
2.9
通讯作者：
Kaneko, T
中科院分区：
文献类型：
--
作者：
Cho, RH;Segawa, S;Kaneko, T
为了研究皮质运动控制，我们在本报告中研究了锥体神经元的电特性，并在随附的报告中研究了锥体神经元与皮质脊髓神经元的柱内或柱旁联系。在大鼠运动感觉皮层(FL、HL和M1区)的脑片上记录到锥体神经元，并对其进行染色，其中许多皮质脊髓神经元被逆行标记。它们在形态上可分为经典锥体神经元、星形神经元和其他改良型锥体神经元，并根据对500ms去极化电流脉冲的反应模式从电生理学上分为规则峰(RS)神经元、内源性爆发(IB)神经元和不规则峰(IS)神经元。RS反应分为慢适应RS(RS-SA)和快适应RS(RS-FA)。电学特性与神经元的板层位置有关，RS-SA反应多见于II/III层，较少见于IV-VI层，IB和IS反应仅见于V层和VI层。有趣的是，FL/HL区的IV层神经元都是RS-FA星形锥体神经元，而MI区的IV层神经元是RS-SA经典锥体神经元。虽然已知弱刺激FL/HL和M1区可引起运动，但这些结果提示这两个区之间的信息处理方式不同。(C)2004年爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
To study cortical motor control, we examined electrical characteristics of pyramidal neurons in the present report, and intra- or juxta-columnar connections of the pyramidal neurons to corticospinal neurons in the accompanying report. Pyramidal neurons were intracellularly recorded and stained in slices of rat motorsensory cortices (areas FL, HL and M1) where many corticospinal neurons were labeled retrogradely. They were morphologically classified into classical, star and other modified pyramidal neurons, and electrophysiologically into regular-spiking (RS), intrinsic bursting (IB) and irregular-spiking (IS) neurons on the basis of spiking pattern in response to 500 ms depolarizing current pulses. RS responses were further divided into RS with slow adaptation (RS-SA) and RS with fast adaptation (RS-FA). The electrical properties were associated with the laminar location of the neurons; RS-SA responses were observed frequently in layer II/III and less frequently in layers IV-VI, and IB and IS responses were exclusively found in layers V and VI, respectively. Interestingly, all layer IV neurons in area FL/HL were RS-FA star-pyramidal neurons, whereas layer IV neurons in area M I were RS-SA classical pyramidal neurons. Although weak stimulation of areas FL/HL and M1 is known to elicit movement, these results suggest different information processings between the two areas. (C) 2004 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.