Guided Migration of Neural Stem Cells Derived from Human Embryonic Stem Cells by an Electric Field
Guided Migration of Neural Stem Cells Derived from Human Embryonic Stem Cells by an Electric Field
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通过电场引导源自人胚胎干细胞的神经干细胞的迁移
DOI：
10.1002/stem.779
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发表时间：
2012-02-01
期刊：
影响因子：
5.2
通讯作者：
Zhao, Min
中科院分区：
文献类型：
--
作者：
Feng, Jun-Feng;Liu, Jing;Zhao, Min
小直流 (DC) 电场 (EF) 引导啮齿动物神经干细胞 (NSC) 的神经突生长和迁移。然而，这可能取决于物种。因此，在任何可能的临床尝试之前，研究人类 NSC (hNSC) 对 EF 的反应至关重要。为了表征 EF 刺激和引导的 hNSC 迁移，我们从成熟的人类胚胎干细胞系 H9 中衍生出 hNSC。施加低至 16 mV/mm 的小直流 EF，会引起显着的向阴极的定向迁移。场极性的反转可逆转 hNSC 的迁移。趋电/趋电响应既依赖于时间又依赖于电压。迁移方向性和到阴极的距离随着场强的增加而增加。 （Rho 激酶）抑制剂 Y27632 用于增强干细胞的活力，之前有报道称可抑制诱导多能干细胞和神经元中 EF 引导的定向迁移。然而，它的存在并没有显着影响 EF 中 hNSC 迁移的方向性。细胞因子受体 [C-X-C 趋化因子受体 4 型 (CXCR4)] 对于大脑中 NSC 的趋化作用很重要。 CXCR4的阻断并不影响hNSC的趋电性。我们得出结论，hNSC 通过定向迁移对小 EF 做出反应。应用的 EF 有可能被进一步利用来引导 hNSC 到达中枢神经系统的损伤部位，以改善各种疾病的结果。干细胞2012； 30：349-355。
Small direct current (DC) electric fields (EFs) guide neurite growth and migration of rodent neural stem cells (NSCs). However, this could be species dependent. Therefore, it is critical to investigate how human NSCs (hNSCs) respond to EF before any possible clinical attempt. Aiming to characterize the EF‐stimulated and guided migration of hNSCs, we derived hNSCs from a well‐established human embryonic stem cell line H9. Small applied DC EFs, as low as 16 mV/mm, induced significant directional migration toward the cathode. Reversal of the field polarity reversed migration of hNSCs. The galvanotactic/electrotactic response was both time and voltage dependent. The migration directedness and distance to the cathode increased with the increase of field strength. (Rho‐kinase) inhibitor Y27632 is used to enhance viability of stem cells and has previously been reported to inhibit EF‐guided directional migration in induced pluripotent stem cells and neurons. However, its presence did not significantly affect the directionality of hNSC migration in an EF. Cytokine receptor [C‐X‐C chemokine receptor type 4 (CXCR4)] is important for chemotaxis of NSCs in the brain. The blockage of CXCR4 did not affect the electrotaxis of hNSCs. We conclude that hNSCs respond to a small EF by directional migration. Applied EFs could potentially be further exploited to guide hNSCs to injured sites in the central nervous system to improve the outcome of various diseases. STEM CELLS 2012; 30:349–355.