Estrous stage- and animal age-independent superovulation in the BrlHan:WIST@Jcl(GALAS) rat
Estrous stage- and animal age-independent superovulation in the BrlHan:WIST@Jcl(GALAS) rat
复制标题
DOI：
10.1538/expanim.54.137
复制
发表时间：
2005-04-01
影响因子：
2.4
通讯作者：
Hioki, K
中科院分区：
文献类型：
--
作者：
Sotomaru, Y;Kamisako, T;Hioki, K
在大多数品系的大鼠中，诱导超数排卵的治疗效果显示出主要的品系差异，并且受动情周期阶段的强烈影响。然而，这项研究表明，通过给予 PMSG 和 hCG，很容易在 Wistar 品系 Brl Han:WIST@Jcl(GALAS) 大鼠中诱导超数排卵。为了证实这种治疗的效果，我们研究了卵子收集效率的年龄差异。间隔48 h腹腔注射150 IU/kg PMSG和75 IU/kg hCG诱导超排卵后，4、8、12、20和28周龄大鼠获得的卵母细胞平均数分别为38.9、33.5、46.1、26.9和21.3。没有观察到由 PMSG 给药时动情阶段引起的差异。在胚胎移植实验中，从每周龄超排卵大鼠获得的受精卵在足月前表现出与未经治疗的大鼠相同的活力。这些结果表明，与发情阶段无关的超排卵不仅在青春期前有效，而且对成年大鼠也有效。
In most strains of rats, the effects of treatment for the induction of superovulation show major strain differences and are strongly influenced by the stage of the estrous cycle. This study demonstrated, however, that superovulation was easily induced in Wistar strain Brl Han:WIST@Jcl(GALAS) rats by PMSG and hCG administration. To confirm the effects of such treatment, we studied age differences in egg collection efficiency. After superovulation was induced by intraperitoneal administration of 150 IU/kg PMSG and 75 IU/kg hCG given 48 h apart, the mean numbers of oocytes obtained from rats at 4, 8, 12, 20 and 28 weeks of age were 38.9, 33.5, 46.1, 26.9 and 21.3, respectively. No differences caused by the estrous stage at the PMSG administration were observed. In an embryo transfer experiment, fertilized eggs obtained from superovulated rats at each week of age showed equivalent viability until full-term to those from untreated rats. These results suggest that estrous stage-independent superovulation is effective not only in the prepubertal stage but also in adult rats.